Nucleotide Analogs - Jena Bioscience
Nucleotide Analogs - Jena Bioscience
Nucleotide Analogs - Jena Bioscience
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Macromolecular Crystallography<br />
102<br />
JBScreen Cryo Pro<br />
JBScreen Cryo Pro is the most convenient tool on the market for<br />
producing effective cryoprotectants from your crystallization reservoir<br />
solution. The kit contains 12 different compounds, divided into sugar/<br />
amino acid-based cryoprotectants, alcohol-based cryoprotectants, and<br />
an oil-based cryoprotectant.<br />
Cat. No. Amount Price (€)<br />
JBScreen Cryo Pro CC-102 1 Kit 150,--<br />
Formulation<br />
Cryo 01 Sucrose / Xylitol<br />
Cryo 02 Sucrose / Glucose<br />
Cryo 03 Sucrose<br />
Cryo 04 Xylitol<br />
Cryo 05 Taurine<br />
Cryo 06 Glycerol<br />
Cryo 07 Pentaerythritol Propoxylate (5/4 PO/OH)<br />
Cryo 08 Pentaerythritol Ethoxylate (15/4 EO/OH)<br />
Cryo 09 Ethylene Glycol<br />
Cryo 10 MPD ((±)-MPD)<br />
Cryo 11 PEG 400<br />
Cryo 12 Mineral Oil<br />
Cryo I & II<br />
Sparse matrices for the crystallization of biological macromolecules.<br />
Every formulation will fl ash-freeze to a clear amorphous glass in liquid<br />
nitrogen or in the cryo-stream at 100 K. Eleven different cryocrystallants<br />
and sparing use of glycerol ensures a broad sampling of possible cryo<br />
conditions. Crystals can be frozen directly from their growth chambers,<br />
thus avoiding the additional step of pre-equilibration with an artifi cial<br />
cryo-solvent that can damage the crystal.<br />
Cryo I & II: 10ml each prepared with ultra-pure chemicals and<br />
water (18.2 Megohm-cm) followed by sterile 0.22 micron fi ltration into<br />
http://www.jenabioscience.com<br />
Selected References:<br />
López-Jaramillo et al. (2002) Soaking: the effect of osmotic shock on tetragonal lysozyme<br />
crystals. Acta Cryst. D58:209.<br />
Kriminski et al. (2002) Flash-cooling and annealing of protein crystals. Acta Cryst. D58:459.<br />
Samygina et al. (2000) Improving the X-ray resolution by reversible fl ash-cooling combined with<br />
concentration screening, as exemplifi ed with Ppase. Acta Cryst. D56:595.<br />
Kwong and Liu (1999) Use of cryoprotectants in combination with immiscible oils for fl ash cooling<br />
macromolecular crystals. J. Appl. Cryst. 32:102.<br />
Riboldi-Tunnicliffe and Hilgenfeld (1999) Cryocrystallography with oil − an old idea revived. J.<br />
Appl. Cryst. 32:1003.<br />
Harp et al. (1998) Macromolecular crystal annealing: Overcoming increased mosaicity<br />
associated with cryocrystallography. Acta Cryst. D54:622.<br />
Garman and Schneider (1997) Macromolecular Cryocrystallography. J. Appl. Cryst. 30:211.<br />
sterile 15 ml tubes. Include 2 ml each of a Light Silicone Oil (DC-200<br />
BDH#630024N) and a Heavy Paraffi n Oil (Fluka #76235) for use in<br />
modulating vapor diffusion rates. US PATENT 6,267,935.<br />
Cryo I & II in 96 Well Matrix Block Plate: The full 96 unique<br />
formulations of the Cryo I and II screens. 1.7ml each prepared with<br />
ultra-pure chemicals and water (18.2 Megohm-cm) followed by sterile<br />
0.22 micron fi ltration into a sterile 96 deep well matrix block plate that is<br />
sealed with a sterile reusable matte cover. US PATENT 6,267,935.<br />
Cryo I (48 conditions)<br />
Cryo II (48 conditions)<br />
Cryo I + II (all 96 reagents)<br />
Cryo I & II<br />
(96 Well Matrix Block Plate)<br />
Cat. No. Amount Price (€)<br />
EBS-<br />
CRYO-I<br />
EBS-<br />
CRYO-II<br />
EBS-<br />
CRYO-F<br />
1 Kit 310,--<br />
1 Kit 310,--<br />
1 + 1 Kit 600,--<br />
EBS-BCY 1 Kit 310,-
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