Nucleotide Analogs - Jena Bioscience

Recombinant Proteins
134
WASP-121 GST
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-369 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 50 mM Tris-
HCl, pH 7.5, 100 mM NaCl, and 2 mM DTE.
The Wiskott-Aldrich syndrome protein (WASP) is the
founding member of a family of proteins that have
emerged as crucial effectors of Rho GTPases and
activators of the cytosceletal-organizing complex
Arp2/3. WASP has been shown to be intimately
involved in many pathways that infl uence the function
of the immune system.
WASP-121 comprises the amino acids 201-321 of the
native WASP sequence. This domain is essential for
the binding of Cdc42 to WASP.
The GST-Tag facilitates the protein`s application in
typical GST pull down assays.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Thrasher A.J. (2002) WASp in immun-system organization and
function. Nature Reviews Immunology 2:635.
Rudolph et al. (1998) The Cdc42/Rac interactive binding region
of the Wiskott-Aldrich-Syndrome Protein (WASP) is necessary
but not suffi cient for tight binding to Cdc42 and structure
formation. J. Biol. Chem. 273:18067.
GTPase Modifying
Enzymes
GGTase-I
rat, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-101 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 25 mM
HEPES, pH 7.2, 25 mM NaCl, and 5 mM DTT.
GGTase (Geranylgeranyltransferase-I) catalyzes the
transfer of the farnesyl and geranylgeranyl groups
from farnesyl and geranylgeranyl-diphosphate to
proteins containing a C-terminal CaaX motif, where
‘C’ is a conserved cysteine that is the site of farnesyl
modifi cation, ‘a’ is usually an aliphatic amino acid, and
‘X’ is leucine or phenylalanine.
Purity: > 90% by SDS-PAGE
Activity: 1 pmol of GGTase-I will transfer 5 pmol of
Farnesyl to RhoA in 15 min at 37°C.
Molecular weight: α: 44 kDa and β: 38 kDa
Store: -80 °C
Selected references:
Yokoyama et al. (1993) Purifi cation of a mammalian protein
geranylgeranyltransferase. Formation and catalytic properties
of an enzyme-geranylgeranyl pyrophosphate complex. J. Biol.
Chem. 268:4055.
GGTase-I GST
rat, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-360 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 100 mM
HEPES, pH 7.2, 100 mM NaCl, and 5 mM DTT.
GGTase (Geranylgeranyltransferase-I) catalyzes the
transfer of the farnesyl and geranylgeranyl groups
from farnesyl and geranylgeranyldiphosphate to
proteins containing a C-terminal CaaX motif, where
‘C’ is a conserved cysteine that is the site of farnesyl
modifi cation, ‘a’ is usually an aliphatic amino acid, and
‘X’ is leucine or phenylalanine.
The GST-Tag facilitates the protein`s application in
typical GST pull-down assays.
Purity: > 90% by SDS-PAGE
Activity: 1 pmol of GGTase-I will transfer 5 pmol of
Farnesyl to RhoA in 15 min at 37°C.
Molecular weight: α: 44 kDa and β: 38 kDa
Store: -80 °C
Selected references:
Yokoyama et al. (1993) Purifi cation of a mammalian protein
geranylgeranyltransferase. Formation and catalytic properties
of an enzyme-geranylgeranyl pyrophosphate complex. J. Biol.
Chem. 268:4055.
GGTase-II (Rab GGTase)
rat, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-103 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 25 mM
HEPES, pH 7.2, 25 mM NaCl, and 5 mM DTT.
GGTase-II (Geranylgeranyltransferase-II) catalyzes
the transfer of geranylgeranyl moiety onto two
C-terminal cysteines of Rab proteins. Composed of
an α and β heterodimer (50 and 38 kDa, respectively)
and requires Rab escort protein for its catalytic
activity. Shown to exhibit higher affi nity towards
geranylgeranyl pyrophosphate (Kd = 8 nM) than
farnesyl pyrophosphate (Kd = 60 nM).
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Kalinin et al. (2001) Expression of mammalian geranylgeranyltransferase
type-II in Escherichia coli and its application
for in vitro prenylation of Rab proteins. Protein Expres. Purif.
22:84.
Thomä et al. (2000) Phosphoisoprenoid binding specifi city of
geranylgeranyltransferase type II. Biochemistry-US 39:12043.
Casey et al. (1996) Protein prenyltransferases. J. Biol. Chem.
271:5289.
FTase
rat, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-102 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 100 mM
HEPES, pH 7.2, 100 mM NaCl, and 5 mM DTT.
FTase catalyzes the transfer of the farnesyl group
from farnesyl diphosphate to proteins containing
a C-terminal CaaX motif, where ‘C’ is a conserved
cysteine that is the site of farnesyl modifi cation, ‘a’ is
usually an aliphatic amino acid, and ‘X’ is methionine,
serine, glutamine, or alanine.
Activity:1 pmol of FTase will transfer 1 pmol of
farnesyl to H-Rasin 15 min at 37°C.
Purity: 90% by SDS-PAGE.
Molecular weight: α: 44 and β: 35 kDa
Store: -80 °C
Selected references:
Zimmerman et al. (1998) High-level expression of rat farnesyl:
pro-teintransferase in Escherichia coli as a translationally
coupled heterodimer. Protein Expres. Purif. 14:395.
http://www.jenabioscience.com
FTase GST
rat, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-361 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 100 mM
HEPES, pH 7.2, 100 mM NaCl, and 5 mM DTT.
FTase catalyzes the transfer of the farnesyl group
from farnesyl diphosphate to proteins containing
a C-terminal CaaX motif, where ‘C’ is a conserved
cysteine that is the site of farnesyl modifi cation, ‘a’ is
usually an aliphatic amino acid, and ‘X’ is methionine,
serine, glutamine, or alanine.
The GST-Tag facilitates the protein`s application in
typical GST pull-down assays.
Activity: 1 pmol of FTase will transfer 1 pmol of
farnesyl to H-Ras in 15 min at 37°C.
Purity: 90% by SDS-PAGE.
Molecular weight: α 44 and β 35 kDa
Store: -80 °C
Selected references:
Zimmerman et al. (1998) High-level expression of rat farnesyl:
proteintransferase in Escherichia coli as a translationally
coupled heterodimer. Protein Express. Purif. 14:395.
REP-1 His
(Rab escort protein)
Rat, Recombinant, Sf9 insect cells
Cat. No. Amount Price (€)
PR-105 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 25 mM
HEPES, pH 7.2, 40 mM NaCl, and 5 mM DTT.
REP-1 (Rab escort protein-1) binds to a wide range of
RabGGTase with nanomolar affi nities and supports the
prenylation of RabGTPase mediated by RabGGTase.
Activity: 1 pmol of REP-1 will mediate transfer of
2 pmol of geranylgeraniol by RabGGTase onto 1 pmol
of Rab7 in 3 minutes.
Store: -80 °C
Selected references:
Seabra et al. (1995) Defi cient geranylgeranylation of Ram/
Rab27 in choroideremia. J. Biol. Chem. 270:24420.
Alexandrov et al. (1994) Rab escort protein-1 is a multifunctional
protein that accompanies newly prenylated rab proteins to their
target membranes. EMBO J. 13:5262.
REP-2 His
(Rab escort protein)
human, Recombinant,
Saccharomyces cerevisiae
Cat. No. Amount Price (€)
PR-104 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 25 mM
HEPES, pH 7.2, 40 mM NaCl, and 5 mM DTT.
REP-2 (Rab escort protein-2) binds to a wide range of
RabGGTase with nanomolar affi nities and supports the
prenylation of RabGTPase mediated by RabGGTase,
with an exception of Rab27.
Activity:
1 pmol of REP-2 will mediate transfer of 2 pmol of
geranylgeraniol by RabGGTase onto 1 pmol of Rab7
in 3 minutes.
Purity: > 90% by SDS-PAGE
Store: -80 °C
Selected references:
Cremers et al. (1994) REP-2, a Rab escort protein encoded by
the choroideremia-like gene. J. Biol. Chem. 269:2111.