Nucleotide Analogs - Jena Bioscience

Recombinant Proteins
132
Selected references:
Wedlich-Soldner et al. (2003) Spontaneous cell polarization
through Actomyosin-based delivery of the Cdc42 GTPase.
Science 299:1231.
Zhang et al. (2001) Oligomerization of Rac1 GTPase mediated
by carboxy-terminal polybasic domain. J. Biol. Chem. 276:
8958.
Zhang et al. (1999) A built-in arginine fi nger triggers the selfstimulatory
GTPase-activating activity of Rho family GTPases.
J. Biol. Chem. 274:2609.
Zhang B and Zheng Y (1998) Negative regulation of Rho family
GTPases Cdc42 and Rac2 by homodimer formation. J. Biol.
Chem. 273:25728.
Chamberlain et al. (2004) The p85α Subunit of
Phosphatidylinositol 3’-Kinase Binds to and Stimulates the
GTPase Activity of Rab Proteins. J. Biol. Chem. 279:48607.
Rac1 ∆C
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-303 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 30 mM Tris-
HCl, pH 7.0, and 5 mM DTE.
Rho family GTPases Rac1 and Cdc42 belong to
the Ras superfamily of small GTP-binding proteins.
Rac1 is involved in regulation of a variety of signal
transduction processes, ranging from cell growth
to cytoskeletal organisation required for cell motility
and cell adhesion. It has been shown that Rac1 is
inhibited by 6-thioguanine triphosphate (6-Thio-GTP),
a metabolite generated by the immunosuppressive
and anti-infl ammatory drug Azathioprine.
The C-terminal deletion of fi ve amino acids of Rac1∆C
(aa 1-187) includes arginine 188 which is part of the
polybasic domain consisting of six contiguous basic
amino acids. Deletion or mutation of the polybasic
residues decrease the intrinsic GTPase activity and
result in a loss of the self-stimulatory GAP activity.
Protein preparation is 100% GDP-loaded, measured
by HPLC.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Zhang et al. (2001) Oligomerization of Rac1 GTPase mediated
by carboxy-terminal polybasic domain. J. Biol. Chem. 276:
8958.
Knaus et al. (1998) Structural requirements for PAK activation
by Rac GTPases. J. Biol. Chem. 273:21512.
Tiede et al. (2003) CD28-dependent Rac1 activation is the
molecular target of azathioprine in primary human CD4 + T
lymphocytes. J. Clin. Invest. 111:1133.
Chamberlain et al. (2004) The p85α Subunit of
Phosphatidylinositol 3’-Kinase Binds to and Stimulates the
GTPase Activity of Rab Proteins. J. Biol. Chem. 279:48607.
RhoA
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-106 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 25 mM
HEPES, pH 7.2, 40 mM NaCl, 5 mM DTT, 2 mM
MgCl 2, and 10 mM GDP.
RhoA is a small GTPase of the Ras superfamily and
regulates stress fi ber formation in response to growth
factor stimulation.
Purity: > 90% by SDS-PAGE
Store: -80 °C
Selected references:
Ridley (1996) Rho: Theme and variation. Curr. Biol. 6 :1256.
Ridley et al. (1992) The small GTP-binding protein Rho
regulates the assembly of focal adhesions and actin stress
fi bers in response to growth factors. Cell 70:389.
RhoA GST
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-363 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 25 mM
HEPES, pH 7.2, 40 mM NaCl, 5 mM DTT, 2 mM
MgCl 2 , and 10 mM GDP.
RhoA is a small GTPase of the Ras superfamily and
regulates stress fi ber formation in response to growth
factor stimulation.
The GST-Tag facilitates the protein`s application in
typical GST pull-down assays.
Purity: > 90% by SDS-PAGE
Store: -80 °C
Selected references:
Ridley (1996) Rho: Theme and variation Curr. Biol. 6:1256.
Ridley et al. (1992) The small GTP-binding protein Rho
regulates the assembly of focal adhesions and actin stress
fi bers in response to growth factors. Cell 70:389.
Guanine Nucleotide
Exchange Factors (GEFs)
Small GTPases are normally membrane
associated independently of their activation
state. Interaction with a specifi c “guanidine
exchange factor” (GEF) induces the GDP –
GTP exchange and thereby activates the
GTPase, allows for interaction with effector
proteins, and localizes and/or activates these
proteins.
EPAC-1 ∆DEP
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-304 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 50 mM
Tris-HCl, pH 7.6, 50 mM NaCl, 5 mM DTE, and 5%
glycerol.
EPAC (exchange protein directly activated by cAMP)
is a Rap-specifi c guanine-nucleotide exchange factor
(GEF). EPAC is activated by the binding of cAMP to a
cyclic nucleotide monophosphate-binding domain.
N-terminal deletion of the DEP domain (domain that
occur in Dishevelled, Egl-10, and Pleckstrin) of EPAC
∆DEP does not affect regulation of EPAC-activity but
affects membrane localization.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Qiao et al. (2002) Cell cycle-dependent subcellular localization
of exchange factor directly activated by cAMP. J. Biol. Chem.
277:26581.
Krämer et al. (2001) Dynamic interaction of cAMP with the Rap
guanine-nucleotide exchange factor EPAC1. J. Mol. Biol. 306:
1167.
de Rooij et al. (2000) Mechanism of regulation of the EPAC
family of cAMP-dependent Rap GEFs. J. Biol. Chem. 275:
20829.
de Rooij et al. (1998) Epac is a Rap1 guanine-nucleotideexchange
factor directly activated by cAMP. Nature 396:474.
http://www.jenabioscience.com
RalGDS-RBD GST
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-365 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 30 mM Tris-
HCl, pH 7.5, 100 mM NaCl, 5 mM MgCl 2 , and 5 mM
DTE.
Ras-binding domain (RBD) of the human RalGDS,
comprises amino acids 1-97 of wild type.
RalGDS (Ral-guanine nucleotide dissociation
stimulator) is the guanine nucleotide exchange factor
(GEF) for the Ras-related protein Ral. The GEFactivity
of RalGDS is stimulated by binding of Ras.
The GST-Tag facilitates the protein`s application in
typical GST pull down assays.
Purity: > 90% by SDS-PAGE
Store: -80 °C
Selected references:
Vetter et al. (1999) Structural and biochemical analysis of Raseffector
signaling via RalGDS. FEBS Letters 451:175.
Murphy et al. (2002) Involvement of phosphatidylinositol
3-kinase, but not RalGDS, in TC21/R-Ras2-mediated
transformation. J. Biol. Chem. 277:9966.
RCC1 (RanGEF), wild type
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-306 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 20 mM
HEPES, pH 7.6, 50 mM NaCl, and 2 mM DTE.
RCC1 (Regulator of Chromosome Condensation,
a β propeller chromatin-bound protein) catalyses
guanine nucleotide exchange of the Ras-related
nuclear protein Ran. RCC1 is the guanine nucleotide
exchange factor (GEF) for Ran which induces
exchange between the bound GDP and cellular GTP.
Genetic studies have suggested that RCC1 may be
involved in sensing the replication state of DNA and
controlling the activation of Cdc2/cyclin B protein
kinase through Ran.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Renault et al. (2001) Structural Basis for Guanine Nucleotide
Exchange on Ran by the regulator of Chromosome
Condensation (RCC1). Cell 105:245.
Clarke et al. (1995) Regulation of Cdc2/cyclin B activation by
Ran, a Ras-related GTPase. J. Cell Sci. 108:1217.
Bischoff FR and Ponstingl H (1991) Catalysis of guanine
nucleotide exchange on Ran by the mitotic regulator RCC1.
Nature 354:80.