Nucleotide Analogs - Jena Bioscience

RabGDI
bovine, Recombinant, Sf9
insect cells
Cat. No. Amount Price (€)
PR-110 50 µg 180,--
Liquid. Supplied as 1 mg/ml solution in 40 mM HEPES,
pH 7.2, 50 mM NaCl, 100 µM GDP and 3 mM DTE.
Rab GDP Dissociation Inhibitor (RabGDI) forms
complexes with cytosolic prenylated GDP-bound Rab
proteins and delivers them to the target membrane.
It also retreaves GDP-bound Rab proteins from the
membrane releases.
Purity: ≥ 90% by SDS-PAGE.
Store: -80 °C
Selected references:
Horiuchi et al. (1995) A GDP/GTP exchange-stimulatory activity
for the Rab5-RabGDI complex on clathrin-coated vesicles from
bovine brain. J. Biol. Chem. 270:11257.
Ullrich et al. (1993) Rab GDP dissociation inhibitor as a general
regulator for the membrane association of rab proteins. J. Biol.
Chem. 168:18143.
Ran Family
Ran (Ras-related nuclear protein) is involved
in diverse cellular processes like nucleo-cytosplasmic
transport of proteins into the nucleus
and RNA into the cytoplasm, mitotic spindle assembly,
and post-mitotic nuclear assembly. The
Ran-regulated nucleo-cytoplasmic transport
through the nuclear core complex occurs in
conjunction with importins and exportins.
Ran
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-211 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 20 mM
KH 2 PO 4 /K 2 HPO 4 , pH 7.0, 6.5 mM DTE, 150 mM KCl,
5 mM MgCl 2 , and 5% glycerol.
Ran (Ras-related nuclear protein, TC4) belongs to the
superfamily of GTP binding proteins. It acts in diverse
cellular processes such as nuclear transport, mitotic
spindle assembly, and post-mitotic nuclear assembly.
Protein preparation is 90% GDP-loaded, measured
by HPLC.
Purity: > 95% by SDS-PAGE
Store: -80°C
Selected references:
Dasso M (2002) The Ran GTPase: Theme and Variations.
Current Biology 12:502.
Renault et al. (2001) Structural Basis for Guanine Nucleotide
Exchange on Ran by the regulator of Chromosome
Condensation (RCC1). Cell 105:245.
Ran (T24N)
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-212 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 20 mM
KH 2 PO 4 /K 2 HPO 4 , pH 7.0, 6.5 mM DTE, 150 mM KCl,
5 mM MgCl 2 , and 10% glycerol.
Ran (Ras-related nuclear protein, TC4) belongs to the
superfamily of GTP binding proteins. It acts in cellular
processes such as nuclear transport, mitotic spindle
assembly, and post-mitotic nuclear assembly. The
mutation T24N results in no or little binding to GTP
and is known to act as a negative inhibitor of nuclear
import.
Protein preparation is 18% GDP-loaded, measured
by HPLC.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Dasso M (2002) The Ran GTPase: Theme and Variations.
Current Biology 12:502.
Renault et al. (2001) Structural Basis for Guanine Nucleotide
Exchange on Ran by the regulator of Chromosome
Condensation (RCC1). Cell 105:245.
Sekimoto et al. (1996) Interferon-gamma-dependent nuclear
import of Stat1 is mediated by the GTPase activity of Ran/TC4
J. Biol. Chem. 271:31017.
Ran (Q69L)
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-213 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 50 mM
KH 2PO 4/K 2HPO 4, pH 7.5, 5 mM DTE, and 5 mM
MgCl 2 .
Ran (Ras-related nuclear protein, TC4) belongs to the
superfamily of GTP binding proteins. It acts in diverse
cellular processes such as nuclear transport, mitotic
spindle assembly, and post-mitotic nuclear assembly.
Ran Q69L is defective in GTP hydrolysis. Useful as a
negative control for Ran binding and import studies.
Protein preparation is 34% GDP- and 17% GTPloaded,
measured by HPLC.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Dasso (2002) The Ran GTPase: Theme and Variations. Current
Biology 12:502.
Palacios et al. (1996) Ran/TC4 mutants identify a common
requirement for snRNP and protein import into the nucleus. J.
Cell Biol. 133:485.
Clarke et al. (1995) Regulation of Cdc2/cyclin B activation by
Ran, a Ras-related GTPase. J. Cell Sci. 108:1217.
Ran (G19V/Q69L)
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-214 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 50 mM
KH 2PO 4/K 2HPO 4, pH 7.0, 6.5 mM DTE, 150 mM KCl,
and 5 mM MgCl 2.
Ran (Ras-related nuclear protein, TC4) belongs to
the Ras superfamily of GTP binding proteins. It acts
in cellular processes such as nuclear transport, mitotic
spindle assembly, and post-mitotic nuclear assembly.
The mutation Q69L abolishes GTP hydrolysis. The
mutation G19V results in a gain-of-function mutant
that is not, however, sensitive to the exchange factor
RCC1.
Protein preparation is 19% GDP-loaded, measured
by HPLC.
Purity: > 95% by SDS-PAGE
Store: -80 °C
GTP Signal Transduction
Selected references:
Siebrasse et al. (2002) Reconstitution of nuclear protein export
in isolated nuclear envelopes. J. Cell Biol. 158:849.
Lounsbury K.M. (1996) Mutations within Ran/TC4 GTPase.
Effects on regulatory factor interactions and subcellular
localization. J. Biol. Chem. 271:32834.
Palacios et al. (1996) Ran/TC4 mutants identify a common
requirement for snRNP and protein import into the nucleus. J.
Cell Biol. 133:485.
Clarke et al. (1995) Regulation of Cdc2/cyclin B activation by
Ran, a Ras related GTPase. J. Cell Sci. 108:1217.
Ran (E70A)
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-215 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 20 mM
KH 2 PO 4 /K 2 HPO 4 , pH 7.4, 1 mM DTE, and 5 mM
MgCl 2 .
Ran (Ras-related nuclear protein, TC4) belongs to
the Ras superfamily of GTP binding proteins. It acts
in cellular processes such as nuclear transport, mitotic
spindle assembly, and post-mitotic nuclear assembly.
The mutation E70A results in inhibition of the guanine
exchange reaction mediated by RCC1, the guanine
nucleotide exchange factor (GEF) for Ran.
Protein preparation is 85% GDP-loaded, measured
by HPLC.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Dasso M (2002) The Ran GTPase: Theme and Variations.
Current Biology 12:502.
Renault et al. (2001) Structural Basis for Guanine Nucleotide
Exchange on Ran by the regulator of Chromosome
Condensation (RCC1). Cell 105:245.
Rho/Rac/Cdc42 Family
GTPases of the Rho/Rac/Cdc42 family interact
with cellular target proteins to regulate a variety
of cellular responses, including apoptosis,
migration, contraction, stress response,
cell growth control, infl ammation, malignant
transformation, and gene transcription.
Cdc42 ∆C
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-302 50 µg 180,--
Liquid. Supplied as a 1 mg/ml solution in 30 mM Tris-
HCl, pH 7.0, and 5 mM DTE.
Rho family GTPases Rac1 and Cdc42 (cell division
cycle 42) belong to the Ras superfamily of small
GTP-binding proteins. The human homolog of yeast
Cdc42 is essential for cell polarity and regulates
cytoskeletal rearrangements in responses to growth
factor stimulation. The C-terminal deletion of 13 amino
acids of Cdc42∆C includes the polybasic domain
consisting of six contiguous basic amino acids. The
polybasic domain of Cdc42 is required for homodimer
formation.
Protein preparation is 100% GDP-loaded, measured
by HPLC.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Recombinant Proteins
131