Nucleotide Analogs - Jena Bioscience

High-level inducible expression of target proteins in LEXSY
Use of heterologous T7 polymerase-TET repressor system for high-level protein synthesis
Uncoupling of target gene expression from host regulatory network for overexpression
Expression Starter Kits – the easiest way to establish LEXSY in your laboratory
Product Cat. No. Amount Price (€)
Inducible LEXSY Starter Kit
bleomycin selection
(contains vector pTUBAPX1.4ble)
Inducible LEXSY Starter Kit
neomycin selection
(contains vector pTUBAPX1.4neo)
EGE-120 1 Kit 2250,--
EGE-121 1 Kit 2250,--
Each kit contains:
- L. tarentolae T7-TR host strain expressing T7 polymerase and TET repressor as live suspension culture
- 50 ml LEXSY Broth BHI - ready-to-go for initial inoculations
- Components for preparation of 1 L LEXSY Broth BHI
- Expression vector of choice with respective selection marker
- The respective antibiotic for 1 L medium
- 50 ml electroporation buffer (LV-HEB)
- Primer sets for insert sequencing and diagnostic PCR
- Manual with detailed description.
The components of these kits are also available separately.
Important Notice for LEXSY Kit Customers:
Upon arrival of the kit, please process the live Leishmania tarentolae host strain culture immediately as described in the manual and transfer the kit components to
the temperatures indicated. Failure of immediate processing as described may result in death of Leishmania cells.
Expression vectors for inducible expression of target proteins in LEXSY
Product Cat. No. Amount Price (€)
LEXSY Inducible Expression
Vector pTUBAPX1.4ble
(bleomycin selection)
LEXSY Inducible Expression
Vector pTUBAPX1.4neo
(neomycin selection)
EGE-220 5 µg 480,--
EGE-221 5 µg 480,--
Expression vectors were designed for stable integration into host tubulin
locus following linearization with PacI and PmeI.
Simply transfect host strain T7-TR expressing T7 polymerase and TET
repressor (LT-111) with your linearized expression plasmid.
Induction of target protein expression is performed by addition of 1 mg/L
tetracyclin.
- Convenient insertion of target gene (X) by stuffer fragment replacement
- Stable integration of expression cassette into host tubulin locus
- Inducible transcription in T7 polymerase/TET repressor strain T7-TR
Inducible protein expression in LEXSY with TET-repressor controlled T7 polymerase
pTUBAPX1.4
8 kbp
X
LEXSY
These Starter Kits contain all essential components to engineer
your inducible LEXSY.
Simply transfect provided host expressing T7 polymerase and
TET repressor with your expression plasmid.
Induction of target protein expression is performed by addition of
1 mg/L tetracyclin.
Pmel
Pacl
EcoRI
PT7op BgIII
BamHI
For host strain maintainment use optimized
LEXSY antibiotics:
LEXSY NTC (Nourseothricin) T7 polymerase
LEXSY Hygro (Hygromycin) TET repressor
For selection of recombinant L. tarentolae
strains use optimized LEXSY antibiotics:
LEXSY Bleo (Bleomycin) for ble vector
LEXSY Neo (Neomycin) for neo vector
LEXSY
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