Nucleotide Analogs - Jena Bioscience

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Formulation Cryo I # Precipitant Buffer (100 mM) Salt/Additives Final pH 1 40% (v/v) MPD Phosphate-citrate pH 4.2 none 5.0 2 40% (v/v) ethylene glycol Acetate pH 4.5 none 5.0 3 50% (v/v) PEG-200 Citrate pH 5.5 none 6.9 4 40% (v/v) PEG-300 HEPES pH 7.5 0.2 M NaCl 7.3 5 40% (v/v) PEG-400 Citrate pH 5.5 0.2 M MgCl2 4.6 6 40% (v/v) PEG-600 Cacodylate pH 6.5 0.2 M Ca(OAc) 2 7.0 7 40% (v/v) ethanol Tris pH 8.5 0.05 M MgCl2 8.4 8 35% (v/v) 2-ethoxyethanol Cacodylate pH 6.5 none 6.9 9 35% (v/v) 2-propanol Phosphate-citrate pH 4.2 none 4.9 10 45% (v/v) glycerol Imidazole pH 8.0 none 7.6 11 35% (v/v) MPD Tris pH 8.5 0.2 M (NH ) SO 4 2 4 8.3 12 50% (v/v) ethylene glycol Acetate pH 4.5 5% (w/v) PEG-1000 5.1 13 30% (v/v) PEG-200 MES pH 6.0 5% (w/v) PEG- 3000 6.1 14 20% (v/v) PEG-300, 10% (v/v) glycerol Phosphate-citrate pH 4.2 0.2 M (NH ) SO 4 2 4 4.5 15 50% (v/v) PEG-400 CHES pH 9.5 0.2 M NaCl 9.2 16 30% (v/v) PEG-600, 10% (v/v) glycerol MES pH 6.0 5% (w/v) PEG- 1000 6.2 17 40% (v/v) 1,2-propanediol HEPES pH 7.5 none 7.2 18 35% (v/v) 2-ethoxyethanol Imidazole pH 8.0 0.05 M Ca(OAc) 2 7.5 19 35% (v/v) 2-propanol Tris pH 8.5 none 8.3 20 30% (v/v) 1,2-propanediol Citrate pH 5.5 20% (v/v) MPD 6.4 21 40% (v/v) 1,2-propanediol Acetate pH 4.5 0.05 M Ca(OAc) 2 5.3 22 40% (v/v) ethylene glycol Na/K phosphate pH 6.2 none 6.8 23 40% (v/v) MPD Tris pH 7.0 0.2 M (NH ) SO 4 2 4 7.3 24 40% (v/v) PEG-400 Na/K phosphate pH 6.2 0.2 M NaCl 6.9 25 30% (v/v) PEG-200 Tris pH 8.5 0.2 M (NH ) HPO 4 2 4 8.7 26 40% (v/v) PEG-300 CHES pH 9.5 0.2 M NaCl 9.4 27 30% (v/v) PEG-400, 10% (v/v) glycerol CAPS pH 10.5 0.5 M (NH ) SO 4 2 4 8.2 28 30% (v/v) PEG-600, 10% (v/v) glycerol HEPES pH 7.5 0.05 M Li SO 2 4 7.4 29 40% (v/v) PEG-300 CHES pH 9.5 0.2 M sodium citrate 9.3 30 35% (v/v) 2-ethoxyethanol Citrate pH 5.5 none 6.3 31 35% (v/v) 2-propanol Citrate pH 5.5 5% (w/v) PEG-1000 6.2 32 40% (v/v) 1,2-propanediol CHES pH 9.5 0.2 M sodium citrate 9.3 33 25% (v/v) 1,2-propanediol, 10% (v/v) glycerol Imidazole pH 8.0 0.2 M Zn(OAc) 2 5.9 34 40% (v/v) MPD Imidazole pH 8.0 0.2 M MgCl2 7.6 35 40% (v/v) ethylene glycol HEPES pH 7.5 5% (w/v) PEG-3000 7.4 36 50% (v/v) PEG-200 Tris pH 7.0 0.05 M Li SO 2 4 6.7 37 40% (v/v) PEG-300 Cacodylate pH 6.5 0.2 M Ca(OAc) 2 7.0 38 40% (v/v) PEG-400 Tris pH 8.5 0.2 M Li SO 2 4 8.4 39 40% (v/v) PEG-600 Phosphate-citrate pH 4.2 none 5.4 40 40% (v/v) ethanol Phosphate-citrate pH 4.2 5% (w/v) PEG-1000 5.2 41 25% (v/v) 1,2-propanediol, 10% (v/v) glycerol Phosphate-citrate pH 4.2 5% (w/v) PEG-3000 4.9 42 40% (v/v) ethylene glycol Tris pH 7.0 none 7.4 43 50% (v/v) ethylene glycol Tris pH 8.5 0.2 M MgCl2 8.5 44 50% (v/v) PEG-200 Cacodylate pH 6.5 0.2 M Zn(OAc) 2 6.2 45 20% (v/v) PEG-300, 10% (v/v) glycerol Tris pH 8.5 5% (w/v) PEG-8000 8.7 46 40% (v/v) PEG-400 MES pH 6.0 5% (w/v) PEG-3000 6.2 47 50% (v/v) PEG-400 Acetate pH 4.5 0.2 M Li SO 2 4 5.1 48 40% (v/v) PEG-600 Imidazole pH 8.0 0.2 M Zn(OAc) 2 5.8 --- Oil 1: Light Silicone DC-200 BDH #6302-4N --- Oil 2: Heavy Paraffi n Fluka #76325 Cryo II Screens # Precipitant Buffer (100 mM) Salt/Additives Final pH 1 40% (v/v) MPD Cacodylate pH 6.5 5% (w/v) PEG-8000 7.0 2 50% (v/v) PEG-200 CHES pH 9.5 none 9.6 3 40% (v/v) ethylene glycol Phosphate-citrate pH 4.2 0.2 M (NH ) SO 4 2 4 4.5 4 40% (v/v) PEG-400 HEPES pH 7.5 0.2 M Ca(OAc) 2 7.2 5 40% (v/v) PEG-300 Tris pH 7.0 5% (w/v) PEG-1000 5.4 6 30% (v/v) PEG-600, 10% (v/v) glycerol cacodylate pH 6.5 1 M NaCl 6.6 7 40% (v/v) ethanol Tris pH 7.0 none 6.9 8 35% (v/v) 2-ethoxyethanol Na/K phosphate pH 6.2 0.2 M NaCl 6.7 9 35% (v/v) 2-propanol Imidazole pH 8.0 0.2 M Zn(OAc) 2 6.1 10 40% (v/v) 1,2-propanediol Acetate pH 4.5 none 5.0 11 25% (v/v) 1,2-propanediol, 10% (v/v) glycerol Na/K phosphate pH 6.2 none 6.8 12 40% (v/v) 1,2-propanediol Citrate pH 5.5 0.2 M NaCl 6.0 13 35% (v/v) MPD Cacodylate pH 6.5 0.05 M Zn(OAc) 2 6.6 14 40% (v/v) ethylene glycol Imidazole pH 8.0 0.2 M Ca(OAc) 2 7.8 15 50% (v/v) PEG-200 Na/K phosphate pH 6.2 0.2 M NaCl 7.2 16 20% (v/v) PEG-300, 10% (v/v) glycerol Imidazole pH 8.0 1 M (NH ) SO 4 2 4 7.4 17 50% (v/v) PEG-400 MES pH 6.0 none 6.3 18 40% (v/v) PEG-300 Phosphate-citrate pH 4.2 none 5.2 19 40% (v/v) PEG-600 Acetate pH 4.5 0.2 M MgCl2 4.8 20 50% (v/v) ethylene glycol CHES pH 9.5 0.5 M K/Na tartrate 9.3 21 35% (v/v) 2-ethoxyethanol Tris pH 8.5 0.2 M Li SO 2 4 8.6 22 35% (v/v) 2-propanol Cacodylate pH 6.5 0.2 M MgCl2 5.9 23 30% (v/v) 1,2-propanediol HEPES pH 7.5 20% (v/v) PEG-400 7.4 24 25% (v/v) 1,2-propanediol, 10% (v/v) glycerol Tris pH 8.5 0.2 M MgCl2 8.5 25 40% (v/v) MPD CAPS pH 10.5 none 10.1 26 40% (v/v) ethylene glycol MES pH 6.0 0.2 M Zn(OAc) 2 6.1 27 50% (v/v) PEG-200 Tris pH 7.0 none 6.8 28 40% (v/v) PEG-300 Imidazole pH 8.0 0.2 M Zn(OAc) 2 5.9 29 30% (v/v) PEG-400, 10% (v/v) glycerol HEPES pH 7.5 5% (w/v) PEG- 3000 30 40% (v/v) PEG-600 Citrate pH 5.5 none 6.5 31 40% (v/v) PEG-600 CHES pH 9.5 none 9.6 32 35% (v/v) 2-propanol Acetate pH 4.5 none 5.3 33 45% (v/v) glycerol Cacodylate pH 6.5 0.2 M Ca(OAc) 2 6.5 34 25% (v/v) 1,2-propanediol, 10% (v/v) glycerol Tris pH 7.0 0.2 M (NH ) SO 4 2 4 7.4 35 40% (v/v) MPD Citrate pH 5.5 none 6.2 36 50% (v/v) PEG-200 Cacodylate pH 6.5 0.2 M MgCl2 6.6 37 50% (v/v) ethylene glycol Imidazole pH 8.0 none 7.7 38 40% (v/v) PEG-400 Acetate pH 4.5 none 5.4 39 30% (v/v) PEG-600, 10% (v/v) glycerol Tris pH 7.0 0.5 M (NH ) SO 4 2 4 5.6 40 40% (v/v) MPD CHES pH 9.5 none 9.2 41 50% (v/v) ethylene glycol HEPES pH 7.5 0.2 M Li SO 2 4 7.3 42 30% (v/v) PEG-200 Acetate pH 4.5 0.1 M NaCl 5.0 43 40% (v/v) PEG-400 Imidazole pH 8.0 none 7.4 44 35% (v/v) MPD, 10% (v/v) glycerol Acetate pH 4.5 none 5.2 45 40% (v/v) PEG-300 Acetate pH 4.5 0.2 M NaCl 5.3 46 30% (v/v) PEG-200 CAPS pH 10.5 0.2 M (NH ) SO 4 2 4 8.7 47 50% (v/v) PEG-200 HEPES pH 7.5 none 7.5 48 50% (v/v) PEG-200 Phosphate-citrate pH 4.2 --- Oil 1: Light Silicone DC-200 BDH #6302-4N --- Oil 2: Heavy Paraffi n Fluka #76325 7.4 0.2 M NaCl 5.2 Macromolecular Crystallography 103
Macromolecular Crystallography 104 Crystallization Optimization JBScreen Plus To select the formulations of the JBScreen Plus solutions, numerous reports on the use of additives to improve the quality and size of macromolecular crystals have been evaluated. These additives are commonly small molecules, which seem to affect sample-sample and sample-solvent interactions, as well as the solvent structure itself. JBScreen Plus comprises 5 kits, including kosmotropic (structurestabilizing) and chaotropic (structure-disturbing) additives, salts, volatile and non-volatile organics and other compounds. Each kit contains 24 different additives, supplied in ready-made aliquots (100 µl each, except the JBScreen Plus Volatiles kit, containing 1 ml each), with adjusted concentration and sterile fi ltered. All solutions come in tightly closing glass vials that will keep them fresh and sterile. JBScreen Plus solutions are most useful in the optimization of preliminary crystallization conditions, where specifi c interactions between the additives and the biological macromolecule may assist in the initial crystallization of the sample and may help to improve the size and diffraction quality of the crystals. Recommended reading • Herberhold et al. (2004) Effects of Chaotropic and Kosmotropic Cosolvents on the Pressure- Induced Unfolding and Denaturation of Proteins: An FT-IR Study on Staphylococcal Nuclease. Biochemistry 43:3336. • Batchelor et al. (2004) Impact of protein denaturants and stabilizers on water structure. J. Am. Chem. Soc. 126:1958. • Boström et al. (2003) Specifi c ion effects: Why the properties of lysozyme in salt solutions follow a Hofmeister series. Biophys. J. 85:686. • Uedaira et al. (2001) Role of hydration of polyhydroxy compounds in biological systems. Cell. Mol. Biol. 47:823. • http://www.lsbu.ac.uk/water/kosmos.html • Cacace et al. (1997) The Hofmeister series: salt and solvent effects on interfacial phenomena. Quarterly Reviews of Biophysics 30:241. • Von Hippel et al. (1965) On the Conformational Stability of Globular Proteins: The Effects of Various Electrolytes and Non-electrolytes on the Thermal Ribonuclease Transition. J. Biol. Chem. 240:3909. Formulation JBScreen Plus Kosmotropic Group Compound M Remarks A 1 Trimethylamine N-oxide 1.00 Zwitterion 2 Proline 1.00 Zwitterion 3 Ectoine 0.10 Zwitterion 4 Glycine Betaine 1.00 Zwitterion 5 Betain monohydrate 0.10 Zwitterion 6 Taurine 0.10 Zwitterion B 1 Glycerol 2.00 Polyalcohol 2 Erythritol 2.00 Polyalcohol 3 Xylitol 2.00 Polyalcohol 4 Adonitol 2.00 Polyalcohol 5 Mannitol 1.00 Polyalcohol 6 Sorbitol 1.00 Polyalcohol C 1 Aluminum Chloride 0.20 Kosmotropic cation 2 Magnesium Chloride 0.20 Kosmotropic cation 3 Calcium Chloride 0.20 Kosmotropic cation 4 Lithium Chloride 2.00 Kosmotropic cation 5 Manganese (II) Chloride 1.00 Kosmotropic cation 6 Zinc Chloride 1.00 Kosmotropic cation D 1 Sodium Malonate 2.00 Kosmotropic anion 2 Sodium Citrate 2.00 Kosmotropic anion 3 Sodium Fluoride 0.50 Kosmotropic anion 4 Ammonium Sulfate 2.00 Kosmotropic anion 5 Ammonium Hydrogen Phosphate 1.00 Kosmotropic anion 6 Ammonium Formate 2.00 Kosmotropic anion http://www.jenabioscience.com Cat. No. Amount Price (€) JBScreen Plus Kosmotropic CS-501 1 Kit 200,-- JBScreen Plus Chaotropic CS-502 1 Kit 200,-- JBScreen Plus Salts CS-503 1 Kit 200,-- JBScreen Plus Additives CS-504 1 Kit 200,-- JBScreen Plus Volatiles CS-505 1 Kit 200,-- JBScreen Plus Chaotropic Group Compound M Remarks A 1 Urea 1.00 Non-ionic 2 DMSO 4.00 Non-ionic 3 DMF 4.00 Non-ionic 4 Pyridine 1.00 Non-ionic 5 ε-Caprolactam 0.50 Non-ionic 6 Phenol 0.10 Non-ionic B 1 Trimethylammonium Chloride 2.00 Chaotropic cation 2 Guanidinium Chloride 2.00 Chaotropic cation 3 Tetramethylammonium Chloride 2.00 Chaotropic cation 4 Cesium Chloride 1.00 Chaotropic cation 5 Rubidium Chloride 1.00 Chaotropic cation 6 Potassium Chloride 1.00 Chaotropic cation C 1 Potassium Thiocyanate 2.00 Chaotropic anion 2 Potassium Cyanate 2.00 Chaotropic anion 3 Potassium Nitrate 1.00 Chaotropic anion 4 Potassium Acetate 1.00 Chaotropic anion 5 Potassium Iodide 1.00 Chaotropic anion 6 Potassium Bromide 1.00 Chaotropic anion D 1 Sodium trichloroacetate 2.00 Chaotropic anion 2 Sodium Thiocyanate 2.00 Chaotropic anion 3 Sodium Perchlorate 2.00 Chaotropic anion 4 Sodium Nitrate 2.00 Chaotropic anion 5 Sodium Iodide 1.00 Chaotropic anion 6 Sodium Bromide 2.00 Chaotropic anion
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JENA BIOSCIENCE Nucleotides and the
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2 Imprint: Design and Layout by: Gr
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Fax Order Form 4 Jena Bioscience Fa
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Terms/Conditions 6 General Business
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8 http://www.jenabioscience.com
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Nucleotide Analogs 10 General Infor
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Nucleotide Analogs 12 Fluorescent C
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Nucleotide Analogs 14 Labeled Amino
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Nucleotide Analogs 16 Labeled Cytid
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Nucleotide Analogs 18 Labeled γ-Am
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Nucleotide Analogs 20 Labeled with
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Nucleotide Analogs 26 Intrinsically
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Nucleotide Analogs 28 Selected refe
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Nucleotide Analogs 30 mant-GTPγS 2
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Nucleotide Analogs 32 http://www.je
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Nucleotide Analogs 34 Labeled Cytid
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Nucleotide Analogs 36 Labeled 8-[(4
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Nucleotide Analogs 38 Non-hydrolyza
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Nucleotide Analogs 40 dATPαS 2‘-
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Nucleotide Analogs 42 Selected refe
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Nucleotide Analogs 44 XTPγS Xantho
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Nucleotide Analogs 46 Bisubstrate I
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Nucleotide Analogs 48 Halogen conta
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Nucleotide Analogs 50 Hei et al. (1
Page 53 and 54: Nucleotide Analogs 52 Amine-labeled
Page 55 and 56: Nucleotide Analogs 54 Other Bases
Page 57 and 58: Nucleotide Analogs 56 dATPαS 2‘-
Page 59 and 60: Nucleotide Analogs 58 γ-Aminohexyl
Page 61 and 62: Nucleotide Analogs 60 Adenosine Nuc
Page 63 and 64: Nucleotide Analogs 62 d4TTP 2’,3
Page 65 and 66: Nucleotide Analogs 64 6-Thio-GTP 6-
Page 67 and 68: Nucleotide Analogs 66 Cyclic Nucleo
Page 69 and 70: Nucleotide Analogs 68 m 7 GTP 7-Met
Page 71 and 72: Nucleotide Analogs 70 XTP Xanthosin
Page 73 and 74: Nucleotide Analogs 72 Vial et al. (
Page 75 and 76: Nucleotide Analogs 74 Non-modifi ed
Page 77 and 78: 76 http://www.jenabioscience.com
Page 79 and 80: Macromolecular Crystallography 78 C
Page 81 and 82: Macromolecular Crystallography 80 J
Page 95 and 96: Macromolecular Crystallography 94 W
Page 97 and 98: Macromolecular Crystallography 96 O
Page 101 and 102: Macromolecular Crystallography 100
Page 103: Macromolecular Crystallography 102
Page 115 and 116: 114 http://www.jenabioscience.com
Page 117 and 118: LEXSY 116 http://www.jenabioscience
Page 119 and 120: LEXSY 118 http://www.jenabioscience
Page 121 and 122: LEXSY 120 Secretory expression of t
Page 123 and 124: LEXSY 122 Synthetic serum- and prot
Page 125 and 126: LEXSY 124 Important licensing infor
Page 127 and 128: Recombinant Proteins 126 GTP Signal
Page 129 and 130: Recombinant Proteins 128 K-Ras 4B h
Page 131 and 132: Recombinant Proteins 130 Rab17 mous
Page 133 and 134: Recombinant Proteins 132 Selected r
Page 135 and 136: Recombinant Proteins 134 WASP-121 G
Page 137 and 138: Recombinant Proteins 136 G sαS -Le
Page 139 and 140: Recombinant Proteins 138 Store: -80
Page 141 and 142: Recombinant Proteins 140 Wenzel-Sei
Page 143 and 144: Recombinant Proteins 142 Mutation o
Page 145 and 146: Recombinant Proteins 144 Naunyn- Sc
Page 147 and 148: Recombinant Proteins 146 Protein co
Page 149 and 150: Recombinant Proteins 148 Membrane P
Page 151 and 152: Recombinant Proteins 150 TBP (TATA
Page 153 and 154: Recombinant Proteins 152 Store: -80
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Recombinant Proteins 154 RAP30 can
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Recombinant Proteins 156 and contai
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Recombinant Proteins 158 BRCA1 (Tum
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Recombinant Proteins 160 Unit defi
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Recombinant Proteins 162 p53, wild
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Recombinant Proteins 164 Sp1 (GC-bo
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Recombinant Proteins 166 p52 (Trans
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Recombinant Proteins 168 Topo I (Hu
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Recombinant Proteins 170 HMG1 (High
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Recombinant Proteins 172 Splicing F
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Recombinant Proteins 174 Lim-Tio et
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Recombinant Proteins 176 GST-LXRα-
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Recombinant Proteins 178 Unit defi
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Recombinant Proteins 180 Purity: >
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Recombinant Proteins 182 PI3 Kinase
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Recombinant Proteins 184 Selected r
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Recombinant Proteins 186 L-α-Phosp
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Recombinant Proteins 188 , Tyrosine
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Recombinant Proteins 190 Purity: >
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Recombinant Proteins 192 p42/ERK2/M
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Recombinant Proteins 194 Akt3/PKBγ
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Recombinant Proteins 196 CK2 substr
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Recombinant Proteins 198 PTP 1B is
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Recombinant Proteins 200 Prion Prot
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Recombinant Proteins 202 Ovine (She
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Recombinant Proteins 204 bovPrPc cD
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Recombinant Proteins 206 HIV-1 Nef
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Recombinant Proteins 208 Antioxidan
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Recombinant Proteins 210 Interleuki
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Enzymes 212 Restriction Enzymes Res
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Enzymes 214 http://www.jenabioscien
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Enzymes 216 Restriction Enzymes Buf
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Enzymes 218 Relative Activity of Re
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Enzymes 220 Acc I 5’...GTMKAC...3
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Enzymes 222 EcoR V 5’...GATATC...
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Enzymes 224 Sca I 5’...AGTACT...3
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Enzymes 226 Selected references: Br
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Enzymes 228 GST-RPB9 (RNA Polymeras
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Enzymes 230 T4 dNMP kinase Bacterio
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Enzymes 232 Topo I Core (Human DNA
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Antibodies 236 Transcription Factor
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Antibodies 238 anti-Dr1 rabbit poly
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Antibodies 240 PI3 Kinase Antibodie
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PCR related Products 244 Standard P
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PCR related Products 246 DNA Mutage
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PCR related Products 248 Polymerase
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PCR related Products 250 Primers an
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PCR related Products 252 DNA Sequen
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PCR related Products 254 50 bp DNA
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Affi nity Chromatography 256 Immobi
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Affi nity Chromatography 260 γ-Ami
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Affi nity Chromatography 264 γ-Ami
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Affi nity Chromatography 268 2’/3
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Index 270 Product Catalog number Pa
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Index 272 Labeled 5-Propargylamino-
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Index 274 Labeled EDA-ATP + 5-FAM..
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Index 276 Labeled γ-Aminooctyl-dUT
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Index 278 Constitutive LEXSY Starte
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Index 280 PrPc (full length, residu
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Index 282 dPTP, L pack ............
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Index 284 Catalog number Product Pa
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Index 286 CC-105 JBScreen Cryo 3 ..
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Index 288 NU-1014L NTP bundle (larg
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Index 290 NU-407S AppNHp (AMPPNP) .
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Index 292 NU-810-540Q Labeled EDA-A
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Index 294 NU-819L γ-Aminoethyl-App
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Index 296 PR-306 RCC1 (RanGEF) ....
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