Nucleotide Analogs - Jena Bioscience

Substrates
L-α-Phosphatidylinositol (PI)
bovine liver, sodium salt
Cat. No. Amount Price (€)
LI-001 5 mg 75,--
H
4
HO
HO
3
H
HO
5
H
OH
2
H
H
6
OH
1
H
O
O
H O
P
O
O
O
O
+ -
Na
O
Powder. Sodium salt.
Phosphatidylinositol is one of the in vitro substrates
for PI3-kinases. Phosphatidylinositol 3-kinase is a
lipid kinase that phosphorylates the inositol ring of
phosphatidylinositol and related compounds at the
3’-OH position.
PI can be used as a substrate for PI3K lipid kinase
activity assay together with a mix of PIP, PIP 2 , PC, PE,
PS and SM.
Molecular weight: 909.12 g/mol
Molecular formula: C 47 H 82 O 13 PNa
Purity: 99%
Store: -20 °C
Selected references:
Vanhaesebroeck et al. (2001) Synthesis and function of 3phosphorylated
inositol lipids. Ann. Rev. Biochem. 70:535.
Balla T. (2001) Pharmacology of phosphoinositides, regulators
of multiple cellular functions. Curr. Pharm. Des. 7:475.
Wymann M.P. (2003) Phosphoinositide 3-kinase signalling
– which way to target? Trend Pharmacol. Sci. 24:323.
Foukas et al. (2002) Direct effects of caffeine and theophylline
on p110 delta and other phosphoinositide 3-kinases. Differential
effects on lipid kinase and protein kinase activities. J. Biol.
Chem. 277:37124
L-α-Phosphatidylinositol-
4-phosphate (PIP)
porcine brain, diammonium salt
Cat. No. Amount Price (€)
LI-002 1 mg 140,--
O H
4
HO
P O
HO
3
+ O
NH - 4
H
HO
5
H
OH
2
O
H O
H
6
H
P
O
O
OH
O
1 + -
NH4 H
O
O
O
Lyophilized. Diammonium salt.
Phosphatidylinositol-4-phosphate is one of the in
vitro substrates for PI3-kinases. Phosphatidylinositol
3-kinase is a lipid kinase that phosphorylates the
inositol ring of phosphatidylinositol-4-phosphate and
related compounds at the 3’-OH position.
PIP can be used as a substrate for PI3K lipid kinase
activity assay together with a mix of PI, PIP 2 , PC, PE,
PS and SM.
Molecular weight: 1001.18 g/mol
Molecular formula: C 47 H 90 N 2 O 16 P 2
Purity: 99%
Store: -20 °C
Selected references:
Vanhaesebroeck et al. (2001) Synthesis and function of 3phosphorylated
inositol lipids. Ann. Rev. Biochem. 70:535.
Balla T. (2001) Pharmacology of phosphoinositides, regulators
of multiple cellular functions. Curr. Pharm. Des. 7:475.
Wymann M.P. (2003) Phosphoinositide 3-kinase signalling
– which way to target? Trend Pharmacol. Sci. 24:323.
Foukas et al. (2002) Direct effects of caffeine and theophylline
on p110 delta and other phosphoinositide 3-kinases. Differential
effects on lipid kinase and protein kinase activities. J. Biol.
Chem. 277:37124.
L-α-Phosphatidylinositol-
4,5-bisphosphate (PIP 2 )
porcine brain, triammonium
salt
HO
Cat. No. Amount Price (€)
LI-003 0.5 mg 140,--
O H
4
HO
P O
O
3
+ O
NH - 4
H
P O
HO
5
+ O
- H
NH4 OH
2
O
H O
H
6
H O
P
O
O
O
OH
O
1 + -
NH O
4 H
Lyophilized. Triammonium salt.
Phosphatidylinositol-4,5-bisphosphate is one of
the in vitro substrates for PI3-kinases and their in
vivo substrate. Phosphatidylinositol 3-kinase is a
lipid kinase that phosphorylates the inositol ring of
phosphatidylinositol and related compounds at the
3‘-OH position.
PIP 2 can be used as a substrate for PI3K lipid kinase
activity assay together with a mix of PI, PIP, PC, PE,
PS and SM.
Molecular weight: 1098.19 g/mol
Molecular formula: C 47 H 94 N 3 O 19 P 3
Purity: 99%
Store: -20 °C
Selected references:
Vanhaesebroeck et al. (2001) Synthesis and function of 3phosphorylated
inositol lipids. Ann. Rev. Biochem. 70:535.
Balla T. (2001) Pharmacology of phosphoinositides, regulators
of multiple cellular functions. Curr. Pharm. Des. 7:475.
Wymann M.P. (2003) Phosphoinositide 3-kinase signalling
– which way to target? Trend Pharmacol. Sci. 24:323.
Foukas et al. (2002) Direct effects of caffeine and theophylline
on p110 delta and other phosphoinositide 3-kinases. Differential
effects on lipid kinase and protein kinase activities. J. Biol.
Chem. 277:37124.
L-α-Phosphatidylcholine (PC)
Chicken egg
Cat. No. Amount Price (€)
LI-004 5 mg 30,--
+
N
O
O
H O
P
O
O
O
O
-
O
Lyophilized.
Phosphatidylcholine is a major phospholipid in the
outer plasma membrane of mammalian cells.
PC together with phosphatidylethanolamine is the
substrate for PS synthase-1 and 2 to synthesize
phosphatidylserine by a base-exchange reaction.
It is used by Phospholipase D to generate the second
messenger phosphatidic acid (PA).
PC can be used in a lipid mix for PI3K lipid kinase
activity assay together with PI, PIP, PIP 2 , PE, PS and
SM.
Molecular weight: 760.09 g/mol
Molecular formula: C 42 H 82 NO 8 P
Purity: 99%
Store: -20 °C
Selected references:
Wymann M.P. (2003) Phosphoinositide 3-kinase signalling
– which way to target? Trend Pharmacol. Sci. 24:323.
Ramoni et al. (2004) Phosphatidylcholine-specifi c phospholipase
C in mitogen-stimulated fi broblasts. Exp. Cell Res. 299:
370.
Farese R.V. (1996) Insulin-sensitive phospholipid signaling
systems and glucose transport: an update. Proc. Soc. Exp.
Biol. Med. 213:1.
Martelli et al. (2004) Metabolism and signaling activities of
nuclear lipids. Cell Mol. Life Sci. 61:1143.
PI3 Kinase Family
L-α-Phosphatidylethanolamine
(PE)
Chicken egg
Cat. No. Amount Price (€)
LI-005 5 mg 30,--
+
N
O
H O
P
O
O
O
-
Lyophilized.
O
O
O
Phosphatidylethanolamine together with phosphatidylcholine
is the substrate for PS synthase-1 and 2 to
synthesize phosphatidylserine by a base-exchange
reaction.
PE can be used in a lipid mix for PI3K lipid kinase
activity assay together with PI, PIP, PIP 2 , PS, PC
and SM.
Molecular weight: 744.05 g/mol
Molecular formula: C 41 H 78 NO 8 P
Purity: 99%
Store: -20 °C
Selected references:
Wymann M.P. (2003) Phosphoinositide 3-kinase signalling
– which way to target? Trend Pharmacol. Sci. 24:323.
Vance J.E. (2003) Molecular and cell biology of
phosphatidylserine and phosphatidylethanolamine metabolism.
Prog. Nucleic Acid Res. Mol. Biol. 75:69.
Recombinant Proteins
185