Nucleotide Analogs - Jena Bioscience

„Caged“ Nucleotides
By esterifi cation of the phosphate moiety nucleotides are rendered biologically
inactive. These ”caged” biomolecules can be introduced into living cells without
evoking any biological response.
Flash photolysis of the caging group by ultraviolet light leads to an ”unmasking”
of the biologically active nucleotide from the inactive caged species resulting in
a rapid and highly localized release of the nucleotide in the cell.
”Caged” nucleotides are increasingly used for:
• Identifi cation of drug targets
• Determination of the affi nity and selectivity of the drug-target interaction
• Identifi cation of the binding site of the target
• Determination of the conformation of the binding pocket in structure-based
drug design
• Monitoring of fast kinetics
• Time-resolved X-ray crystallography
HO
NO 2
CH 3
O
O
O P O P O P O
O
N N
OH OH OH
N
NH
O O O
P O P O P O
OH OH OH
O
N N NH2 O
OH OH
OH OH
O
NPE-caged-GTP
(not biologically active)
N
O
NH
NH 2
h
(350 nm)
GTP
(biologically active)
„Caged“ Nucleotides
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Nucleotide Analogs