Nucleotide Analogs - Jena Bioscience

Recombinant Proteins
192
p42/ERK2/MAPK2 His , inactive
(Extracellular signal-regulated
Kinase/mitogen-activated
protein kinase2)
Rat, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-320 50 µg 160,--
Liquid. Supplied as 1 mg/ml solution in 10 mM
HEPES, pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 1 mM
DTT, and 0.5% n-Octyl-β-D-Glucopyranoside.
Whole sequence with N-terminal His-Tag, inactive.
Extracellular signal-regulated kinase ERK2 is the
key component of the mitogen-activated protein
(MAP) kinase cascade. It plays an essential role in
mitogenesis, differentiation, and cellular transformation
in response to various stimuli such as growth factors.
Purity: > 95% by SDS-PAGE.
Store: -80 °C
Selected reference:
Boulton et al. (1991) ERK´s: a family of protein-serine/threonine
kinases that are activated and tyrosine phosphorylated in
response to insulin and NGF. Cell 65:663.
p44/ERK1/MAPK1 His , active
(extracellular signal-regulated
kinase1/mitogen-activated
protein kinase1)
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-322 2.5 µg 240,--
Supplied in 50 mM Tris-HCl pH 8.5, 150 mM NaCl,
1 mM DTT, 50% glycerol.
N-terminal His-tagged full-length protein.
Members of the MAPK families play an important
role in complex cellular programs like proliferation,
differentiation, development, transformation, and
apoptosis. At least three MAPK families have been
characterized: extracellular signal-regulated kinase
(ERK), Jun kinase (JNK/SAPK) and p38 MAPK.
MAPK pathways relay, amplify and integrate signals
from a diverse range of stimuli and elicit an appropriate
physiological response including cellular proliferation,
differentiation, development, infl ammatory responses
and apoptosis in mammalian cells.
Activated ERK can enter the nucleus and
phosphorylate transcription factors providing the link
between cell surface receptor-mediated events and
nuclear induction of gene expression. In the nucleus
activated ERK promotes the transcription and the
activity of transcription factors c-fos, c-myc, c-jun
and p21.
The recombinant human ERK1 was expressed in
E. coli and purifi ed by Ni-agarose chromatography. It
is suitable for labeling ERK1/MAPK1 substrates. The
highly active form is produced by phosphorylation of
the protein in vitro with MEK1. The sequence based
calculated molecular weight is 43.62 kDa.
Purity: > 95% by SDS-PAGE
Specifi c activity: > 15,000 U/mg (1 Unit is defi ned
as 1 picomole phosphate transferred to myelin basic
protein per min at 30°C)
Store: -80 °C
Selected references:
Zhang W. and Liu H.T. (2002) MAPK signal pathways in the
regulation of cell proliferation in mammalian cells. Cell Res.
12:9.
Ajenjo et al. (2004) Subcellular localization determines
the protective effects of activated ERK2 against distinct
apoptogenic stimuli in myeloid leukemic cells. J. Biol. Chem.
279:32813.
p44/ERK1/MAPK1 His , inactive
(extracellular signal-regulated
kinase/mitogen-activated
protein kinase)
human, Recombinant, E. coli
Cat. No. Amount Price (€)
PR-323 5 µg 160,--
Supplied in 50 mM Tris-HCl pH 8.5, 150 mM NaCl,
1 mM DTT, 50% glycerol.
N-terminal His-tagged full-length protein.
MAPK families play an important role in complex
cellular programs like proliferation, differentiation,
development, transformation, and apoptosis. At
least three MAPK families have been characterized:
extracellular signal-regulated kinase (ERK), Jun
kinase (JNK/SAPK) and p38 MAPK.
MAPK pathways relay, amplify and integrate signals
from a diverse range of stimuli and elicit an appropriate
physiological response including cellular proliferation,
differentiation, development, infl ammatory responses
and apoptosis in mammalian cells.
Activated ERK can enter the nucleus and
phosphorylate transcription factors providing the link
between cell surface receptor-mediated events and
nuclear induction of gene expression. In the nucleus
activated ERK promotes the transcription and the
activity of transcription factors c-fos, c-myc, c-jun
and p21.
The recombinant human ERK1 was expressed in
E. coli. The sequence based calculated molecular
weight is 43.62 kDa.
Purity: > 95% by SDS-PAGE
Store: -80 °C
Selected references:
Zhang W. and Liu H.T. (2002) MAPK signal pathways in the
regulation of cell proliferation in mammalian cells. Cell Res.
12:9.
Ajenjo et al. (2004) Subcellular localization determines
the protective effects of activated ERK2 against distinct
apoptogenic stimuli in myeloid leukemic cells. J. Biol. Chem.
279:32813.
http://www.jenabioscience.com
Calcium/Calmodulin
Dependent Kinases
(CAMK Family)
Free calcium is a major second messenger in
all cell types. One mechanism by which calcium
ions exert their function is by binding to a
17 kDa protein, called calmodulin (CaM). The
binding of four calcium ions to CaM changes its
conformation and thereby activates a number
of other proteins such as various classes of
serine/threonine protein kinases. A practical
way of classifying the calcium/CaM-dependent
protein kinases is based on their substrate specifi
city: some of these enzymes have only one
substrate, and are designated as ‘dedicated’
calcium/CaM-dependent protein kinases, while
others have broad substrate specifi city and are
termed ‘multifunctional’ kinases.
CHK2 His , active
(Checkpoint kinase)
human, Recombinant, Sf9
insect cells
Cat. No. Amount Price (€)
PR-313 20 µg 240,--
Supplied in 50 mM Tris-HCl pH 7.5, 150 mM NaCl,
10 mM DTT, 0.1 mM EGTA, 270 mM sucrose,
protease inhibitor cocktail (Roche).
C-terminal His-tagged full-length protein.
Checkpoint kinase 2 (CHK2) is emerging as a key
mediator of diverse cellular responses to genotoxic
stress, guarding the integrity of the genome
throughout eukaryotic evolution. Recent studies
show the fundamental role of CHK2 in the network of
genome-surveillance pathways that coordinate cellcycle
progression with DNA repair and cell survival or
death. Defects in CHK2 contribute to the development
of both hereditary and sporadic human cancers, and
earmark this kinase as a candidate tumour suppressor
and an attractive target for drug discovery.
The recombinant human CHK2 was expressed in
Sf9 insect cells and purifi ed by Ni-NTA agarose
chromatography. It is suitable for labeling CHK
substrates. The sequence based calculated molecular
weight is 66.06 kDa.
Purity: > 95% by SDS-PAGE
Specifi c activity: > 25,000 U/mg (1 Unit is defi ned as
1 picomole phosphate transferred to synthetic peptide
KKKVSRSGLYRSPSMPENLNRPR (cat.# PE-205)
per min at 30°C)
Store: -80 °C
Selected references:
Bartek et al. (2001) CHK2 kinase--a busy messenger. Nat. Rev.
Mol. Cell Biol. 12:877.
Gire et al. (2004) DNA damage checkpoint kinase Chk2 triggers
replicative senescence. EMBO J. 23:2554.