Molecular Characterization and Gene Expression Profiling ... - CUSAT

Chapter 5
On WSSV challenge, crustin-2 followed the same pattern of expression
as in case of crustin-1. Crustin-2 gene was found to be down-regulated in the
control group of shrimps on challenge with WSSV. However, when
compared to the control group, all experimental diet administered group
supported up-regulation of the crustin-2 gene. CHY administered group was
found to support maximum up-regulation of the crustin-2 gene post-
challenge WSSV, which was followed by CSY, CHG and CSG (Fig. 5.6 B & C).
5.3.2.4. Expression profile of crustin-3
No variations could be detected in the expression profile of crustin-3
gene pre-challenge WSSV, except for a very slight up-regulation in the
treated groups (Fig. 5.7 A & C).
Crustin-3 gene was found to be down-regulated in the control group
of shrimps on WSSV challenge. No variations could be detected in the
expression profile of crustin-3 gene on immunostimulant administration,
post-challenge WSSV (Fig. 5.7 B & C).
5.3.2.5. Expression profile of penaeidin-3
Not much variation in the expression profile of penaeidin-3 could be
detected on administration of immunostimulants pre-challenge WSSV except
for a slight up-regulation in case of marine yeast administered group of
shrimps (Fig. 5.8 A & C).
On WSSV challenge, down-regulation of the penaeidin-3 gene could
be found in the control group of shrimps. However, significant variation in
the expression of penaeidin-3 gene could be observed in the
immunostimulant administered group of shrimps. Maximum up-regulation
of the penaeidin-3 gene was supported by marine yeast administered group,
followed by CSG and CHG administered groups (Fig. 5.8 B & C).
5.3.2.6. Expression profile of penaeidin-5
Not much variation in the expression profile of penaeidin-5 could be
detected on administration of immunostimulants pre-challenge WSSV except
Molecular Characterization and Gene Expression Profiling of Antimicrobial Peptides in Penaeid Shrimps
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