Molecular Characterization and Gene Expression Profiling ... - CUSAT

More documents

Recommendations

Info

(A) (B) -VE CTRL +VE CTRL CHG CSG CHY CSY Chapter 5 Fig. 5.13. Expression profile of latency related gene (WSSV gene) in the haemocytes of giant tiger shrimp, P. monodon in response to the administration of marine whole yeast cell and β-1, 3 glucan as immunostimulants and WSSV challenge (A) Agarose gel electrophoretogram (B) Graphical representation of the expression levels of latency related gene gene (x-axis = immunostimulants used, y-axis = Expression levels of the latency related gene) (-ve control – Unchallenged shrimp, +ve control - WSSV Challenged shrimp, CHY – Candida haemulonii yeast, CSY – Candida sake yeast, CHG – Candida haemulonii glucan, CSG – Candida sake glucan) LATENCY RELATED GENE (647 bp) Molecular Characterization and Gene Expression Profiling of Antimicrobial Peptides in Penaeid Shrimps 324
(A) (B) -VE CTRL +VE CTRL CHG CSG CHY CSY Chapter 5 Fig. 5.14. Expression profile of protein kinase (WSSV gene) in the haemocytes of giant tiger shrimp, P. monodon in response to the administration of marine whole yeast cell and β-1, 3 glucan as immunostimulants and WSSV challenge (A) Agarose gel electrophoretogram (B) Graphical representation of the expression levels of protein kinase gene (x-axis = immunostimulants used, y-axis = Expression levels of the protein kinase gene) (-ve control – Unchallenged shrimp, +ve control - WSSV Challenged shrimp, CHY – Candida haemulonii yeast, CSY – Candida sake yeast, CHG – Candida haemulonii glucan, CSG – Candida sake glucan) PROTEIN KINASE (512 bp) Molecular Characterization and Gene Expression Profiling of Antimicrobial Peptides in Penaeid Shrimps 325
Page 1 and 2:
Molecular Characterization and Gene
Page 3 and 4:
Declaration I hereby do declare tha
Page 5 and 6:
Acknowledgements This work was carr
Page 7 and 8:
My Seniors, Dr. Annies Joseph, Dr.
Page 9 and 10:
Contents Title Page Nos. 1 General
Page 11 and 12:
in response to WSSV challenge 252-2
Page 13 and 14:
α-2M Alpha-2-Macroglobulin ALF Ant
Page 15 and 16:
ppA Prophenoloxidase-Activating Enz
Page 17 and 18:
1.1 Introduction Chapter 1 Aquacult
Page 19 and 20:
Chapter 1 the giant tiger shrimp (P
Page 21 and 22:
Common names: Chapter 1 Giant tiger
Page 23 and 24:
1.3.1. Diseases Chapter 1 Diseases
Page 25 and 26:
Lymphoidal parvo-like virus (LPV) B
Page 27 and 28:
Chapter 1 and protective occlusion
Page 29 and 30:
Chapter 1 small brown masses in the
Page 31 and 32:
1.4.6. Natural epidemics Chapter 1
Page 33 and 34:
Chapter 1 can differentiate WSSV-in
Page 35 and 36:
1.5.1.1. Probiotics Chapter 1 Alter
Page 37 and 38:
Chapter 1 tried in P. monodon, resu
Page 39 and 40:
Chapter 1 The first and essential i
Page 41 and 42:
Chapter 1 1998). The innate immunit
Page 43 and 44:
1.6.1 Haemocytes Chapter 1 The haem
Page 45 and 46:
Chapter 1 In crustaceans, the sheet
Page 47 and 48:
Chapter 1 Theopold et al., 2002). C
Page 49 and 50:
Chapter 1 kill/remove the invading
Page 51 and 52:
Chapter 1 forming complexes with a
Page 53 and 54:
1.7 Antimicrobial Peptides (AMPs) 1
Page 55 and 56:
Chapter 1 the response is very fast
Page 57 and 58:
Chapter 1 Hirsch, 1947). While they
Page 59 and 60:
Chapter 1 AMPs to various degrees u
Page 61 and 62:
1.7.5 Biological activity Chapter 1
Page 63 and 64:
Chapter 1 including the microbes as
Page 65 and 66:
Tachyplesin I Chapter 1 Crustacea P
Page 67 and 68:
Anionic and cationic peptides that
Page 69 and 70:
Anionic and Cationic AMPs with cyst
Page 71 and 72:
Chapter 1 Fig. 1.7. Structural clas
Page 73 and 74:
Chapter 1 Group III: Linear peptide
Page 75 and 76:
Chapter 1 through regular processes
Page 77 and 78:
Chapter 1 ultimately signal to tran
Page 79 and 80:
Chapter 1 divalent polyanionic cati
Page 81 and 82:
Chapter 1 type of transmembrane por
Page 83 and 84:
Chapter 1 other intracellular targe
Page 85 and 86:
Chapter 1 Apidaecin, a short, proli
Page 87 and 88:
Chapter 1 Hultmark, 1987; Gennaro a
Page 89 and 90:
Chapter 1 quite opposite characteri
Page 91 and 92:
Chapter 1 depletion of mitochondria
Page 93 and 94:
Chapter 1 conformations adopted by
Page 95 and 96:
Chapter 1 receptor may be a potenti
Page 97 and 98:
Chapter 1 exposure to their targets
Page 99 and 100:
Chapter 1 generating a rich spectru
Page 101 and 102:
Chapter 1 by enkelytin (Goumon et a
Page 103 and 104:
Lactoferricin Cow Mastitis control
Page 105 and 106:
Chapter 1 trial, in which peptide T
Page 107 and 108:
Chapter 1 a too limited spectrum to
Page 109 and 110:
Chapter 1 number of research groups
Page 111 and 112:
CHAPTER-2 Molecular Characterizatio
Page 113 and 114:
Chapter 2 interaction with negative
Page 115 and 116:
Chapter 2 inhibit the endotoxin or
Page 117 and 118:
Chapter 2 structure be modified to
Page 119 and 120:
Chapter 2 and the region might be l
Page 121 and 122:
Chapter 2 been made of the spectra
Page 123 and 124:
Chapter 2 their discovery and initi
Page 125 and 126:
Chapter 2 terminal pyroglutamic aci
Page 127 and 128:
Chapter 2 One can assume that the p
Page 129 and 130:
Chapter 2 by degranulation into the
Page 131 and 132:
2.2. Materials and Methods 2.2.1. E
Page 133 and 134:
Chapter 2 washed by adding 1 ml 75
Page 135 and 136:
Chapter 2 entry of recombinant DNA
Page 137 and 138:
Chapter 2 and the deduced amino aci
Page 139 and 140:
Chapter 2 Fenneropenaeus, Litopenae
Page 141 and 142:
2.3.1.3. Crustin-1 (GQ334395) Chapt
Page 143 and 144:
2.3.1.3.5. Phylogenetic analysis of
Page 145 and 146:
Chapter 2 sequence also showed high
Page 147 and 148:
Chapter 2 chinensis and F. indicus
Page 149 and 150:
Chapter 2 monodon and Group III of
Page 151 and 152:
Chapter 2 Tiger shrimp (P. monodon)
Page 153 and 154:
Chapter 2 ALF-1 was a partial seque
Page 155 and 156:
Chapter 2 As mentioned before, the
Page 157 and 158:
Chapter 2 Munoz et al., 2004). The
Page 159 and 160:
Table 2.1. Primers used for the stu
Page 161 and 162:
Table 2.4. BLASTn analysis of ALF-2
Page 163 and 164:
Table 2.8. BLASTn analysis of Crust
Page 165 and 166:
Table 2.12. BLASTn analysis of Pena
Page 167 and 168:
Fig.2.5. Agarose gel electrophoreto
Page 169 and 170:
Chapter 2 Fig.2.9. Multiple alignme
Page 171 and 172:
Chapter 2 Fig. 2.12 A bootstrapped
Page 173 and 174:
Chapter 2 Fig. 2.15. Multiple align
Page 175 and 176:
Chapter 2 Fig.2.18. A bootstrapped
Page 177 and 178:
Chapter 2 Fig.2.20. Signal peptide
Page 179 and 180:
Chapter 2 Fig.2.24 Multiple alignme
Page 181 and 182:
Chapter 2 LITOPENAEUS SP. FARFANTEP
Page 183 and 184:
Chapter 2 Fig. 2.28. Nucleotide and
Page 185 and 186:
Page 187 and 188:
Chapter 2 LITOPENAEUS SP. FENNEROPE
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Chapter 2 Fig.2.43 A bootstrapped n
Page 195 and 196:
Page 197 and 198:
Chapter 2 LITOPENAEUS SP. FENNEROPE
Page 199 and 200:
Chapter 2 Fig.2.51 Signal peptide a
Page 201 and 202:
Chapter 2 FENNEROPENAEUS SP. FARFAN
Page 203 and 204:
CHAPTER-3 Molecular Characterizatio
Page 205 and 206:
Chapter 3 certainly help us to unra
Page 207 and 208:
Chapter 3 3.3.1.1. Anti-lipopolysac
Page 209 and 210:
Chapter 3 was predicted out to be 1
Page 211 and 212:
Chapter 3 random coiled structure t
Page 213 and 214:
3.3.1.3.5. Phylogenetic analysis of
Page 215 and 216:
Chapter 3 case has been reported in
Page 217 and 218:
Chapter 3 Multiple alignments were
Page 219 and 220:
Chapter 3 negative bacteria. In shr
Page 221 and 222:
Chapter 3 indicated a random coiled
Page 223 and 224:
Chapter 3 horseshoe crab big defens
Page 225 and 226:
Table 3.4. BLASTn analysis of ALF-2
Page 227 and 228:
Table.3.8. BLASTn analysis of Fi-pe
Page 229 and 230:
Chapter 3 Fig.3.2. Nucleotide and a
Page 231 and 232:
Chapter 3 Fig.3.5. Multiple alignme
Page 233 and 234:
Chapter 3 Fig.3.8. Nucleotide and a
Page 235 and 236:
Chapter 3 Fig.3.12. Multiple alignm
Page 237 and 238:
Chapter 3 Fig.3.14. A bootstrapped
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
SHRIMPS KIND CRAB LOBSTERS CRABS Ch
Page 245 and 246:
Page 247 and 248:
Chapter 3 Fig. 3.28. Multiple align
Page 249 and 250:
PENAEIDIN-2 Chapter 3 PENAEIDIN- 3
Page 251 and 252:
Fig.3.31. Nucleotide and amino acid
Page 253 and 254:
Page 255 and 256:
Chapter 3 Fig.3.37 Nucleotide and a
Page 257 and 258:
4.1. Introduction Chapter 4 The aqu
Page 259 and 260:
Chapter 4 appearance and have major
Page 261 and 262:
Chapter 4 the tissues has recently
Page 263 and 264:
Chapter 4 with the ability of shrim
Page 265 and 266:
Chapter 4 with a low dose of WSSV b
Page 267 and 268:
Chapter 4 molecules they are likely
Page 269 and 270:
Chapter 4 for understanding gene ex
Page 271 and 272:
Chapter 4 4.3.2. Expression profile
Page 273 and 274:
Expression profile of endonuclease
Page 275 and 276:
Chapter 4 bacterial and fungal infe
Page 277 and 278:
Chapter 4 measuring TSV and YHV loa
Page 279 and 280:
Chapter 4 Of particular interest he
Page 281 and 282:
Chapter 4 large distribution and ab
Page 283 and 284:
Chapter 4 WSSV genes viz. endonucle
Page 285 and 286:
Table 4.2. Primers used for the stu
Page 287 and 288:
(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
Page 289 and 290:
(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
Page 291 and 292:
(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
Page 293 and 294: (A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
Page 295 and 296: (A) (B) B 28 1 2 3 4 5 6 7 8 9 10 F
Page 297 and 298: (A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
Page 299 and 300: (A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
Page 301 and 302: (A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
Page 303 and 304: CHAPTER-5 Expression Profile of Ant
Page 305 and 306: Chapter 5 1994). The emergence of b
Page 307 and 308: Chapter 5 (Bovill et al., 2001). In
Page 309 and 310: 5.2.2. Test diets Chapter 5 Four ex
Page 311 and 312: 5.2.2.4. Preparation of Glucan inco
Page 313 and 314: Chapter 5 genes (latency related ge
Page 315 and 316: Chapter 5 On WSSV challenge, crusti
Page 317 and 318: Chapter 5 5.3.4. Expression profile
Page 319 and 320: 5.3.4.5. Expression profile of pena
Page 321 and 322: Chapter 5 must firstly rest on a so
Page 323 and 324: Chapter 5 For the present study two
Page 325 and 326: Chapter 5 ameobocytes of two marine
Page 327 and 328: Chapter 5 variation in expression o
Page 329 and 330: Chapter 5 It has been previously sh
Page 331 and 332: Chapter 5 manifested in terms of gr
Page 333 and 334: (A) (B) (C) C CHY CSY CHG CSG C CHY
Page 341 and 342: (A) (B) -VE CTRL +VE CTRL CHG CSG C
Page 343: (A) (B) -VE CTRL +VE CTRL CHG CSG C
Page 347 and 348: (A) (B) -VE CTRL +VE CTRL CHG CSG C
Page 349 and 350: (A) PRE-CHALLENGE (B) POST-CHALLENG
Page 351 and 352: (A) PRE-CHALLENGE (B) (C) (D) CONTR
Page 353 and 354: (C) (D) (A) PRE-CHALLENGE (B) CONTR
Page 355 and 356: (A) (B) G M HP HR I Chapter 5 Fig.
Page 357 and 358: Chapter 5 Fig. 5.26. Post challenge
Page 359 and 360: 6.1. Introduction Chapter 6 Prophyl
Page 361 and 362: Chapter 6 production of inhibitory
Page 363 and 364: 6.2. Materials and methods 6.2.1. E
Page 365 and 366: Chapter 6 tissue cDNA was performed
Page 367 and 368: Chapter 6 gene was supported by Bac
Page 369 and 370: Chapter 6 treated group of shrimps.
Page 371 and 372: Chapter 6 the gene was found to be
Page 373 and 374: Chapter 6 found to support minimum
Page 375 and 376: Chapter 6 is induced upon bacterial
Page 377 and 378: Chapter 6 all the target genes, exc
Page 379 and 380: Chapter 6 Detailed tissue-wise anal
Page 381 and 382: Chapter 6 Gills and intestine was f
Page 383 and 384: (A) (B) Fig. 6.1. Experimental diet
Page 385 and 386: (A) (B) (C) Fig. 6.3. Expression pr
Page 387 and 388: (A) (B) (C) C B M BM C B M BM Fig.
Page 389 and 390: (A) (B) (C) Fig. 6.7. Expression pr
Page 391 and 392: (A) (B) (C) Chapter 6 Fig. 6.9. Exp
Page 393 and 394: (A) (B) -VE CTRL +VE CTRL B M BM Ch
Page 395 and 396:
(A) (B) -VE CTRL +VE CTRL B M BM Ch
Page 397 and 398:
Page 399 and 400:
Page 401 and 402:
(A) PRE-CHALLENGE (B) POST-CHALLENG
Page 403 and 404:
(A) PRE-CHALLENGE (B) POST-CHALLENG
Page 405 and 406:
(A) (C) (D) PRE-CHALLENGE POST-CHAL
Page 407 and 408:
(A) (B) G M HP HR I Chapter 6 Fig.
Page 409 and 410:
Chapter 6 Fig. 6.27. Post challenge
Page 411 and 412:
7.1. Summary Chapter 7 Tropical shr
Page 413 and 414:
Salient findings Chapter 7 � From
Page 415 and 416:
Chapter 7 � Tissue-wise expressio
Page 417 and 418:
Chapter 7 application in shrimp cul
Page 419 and 420:
References Aboudy, Y., Mendelson, E
Page 421 and 422:
References Anggraeni, M.S., Owens,
Page 423 and 424:
References Bals, R., Wang, X., Zasl
Page 425 and 426:
References derived from the N-termi
Page 427 and 428:
References Breukink, E., de Kruijff
Page 429 and 430:
References Burgents, J.E., Burnett,
Page 431 and 432:
References 1,3-β-D-glucan-binding
Page 433 and 434:
References Chen, J.Y., Chuang, H.,
Page 435 and 436:
References Cole, A.M., Hong, T., Bo
Page 437 and 438:
References Dathe, M., Wieprecht, T.
Page 439 and 440:
References Diamond, G., Zasloff, M.
Page 441 and 442:
References Faber, C., Stallmann, H.
Page 443 and 444:
References Flint, S.J., Enquist, L.
Page 445 and 446:
References Gennaro, R., Zanetti, M.
Page 447 and 448:
References Gudmundsson, G.H., Lidho
Page 449 and 450:
References Harwig, S.S., Swiderek,
Page 451 and 452:
References Hirakura, Y., Kobayashi,
Page 453 and 454:
References Huang, C.C., Song, Y.L.
Page 455 and 456:
References Itami, T., Takahashi, Y.
Page 457 and 458:
References Jiravanichpaisal, P. Whi
Page 459 and 460:
References Kang, J.H., Shin, S.Y.,
Page 461 and 462:
References Kono, T., Savan, R., Sak
Page 463 and 464:
References rich peptide derived fro
Page 465 and 466:
References Li, L., Wang, J.X., Zhao
Page 467 and 468:
References Liu, C.H., Cheng, W., Ku
Page 469 and 470:
References Lorenzon, S., de Guarrin
Page 471 and 472:
References Marone, M., Mozzetti, S.
Page 473 and 474:
References Medvinsky, A., Dzierzak,
Page 475 and 476:
References Moriarty, D.J.W. 1996. M
Page 477 and 478:
References antimicrobial peptide fr
Page 479 and 480:
References Otta, S.K., Karunasagar,
Page 481 and 482:
References simplex virus has heptad
Page 483 and 484:
References infected shrimp. Thesis
Page 485 and 486:
References Litopenaeus vannamei cha
Page 487 and 488:
References Roth, B.L., Poot, M., Yu
Page 489 and 490:
References Sathish, S., Musthaq, S.
Page 491 and 492:
References a proline-arginine-rich
Page 493 and 494:
References Smith, V.J., Fernandes,
Page 495 and 496:
References Song, Y.L., Cheng, W., W
Page 497 and 498:
References experimental system. In:
Page 499 and 500:
References the black tiger shrimp P
Page 501 and 502:
References Tapay, L.M., Cesar, E.,
Page 503 and 504:
References Touhy, K.M., Probert, H.
Page 505 and 506:
References van Hulten, M.C., Reijns
Page 507 and 508:
References Vives, R.R., Imberty, A.
Page 509 and 510:
References Wang, Y.C., Chang, P.S.,
Page 511 and 512:
References Williams, M.J., Rodrigue
Page 513 and 514:
References Wu, W., Zong, R., Xu, J.
Page 515 and 516:
References Yodmuang, S., Tirasophon
Page 517 and 518:
References Zhan, W.B., Wang, Y.H. 1
Page 519 and 520:
Penaeus monodon anti-lipopolysaccha
Page 521 and 522:
Penaeus monodon crustin-like antimi
Page 523 and 524:
Penaeus monodon crustin-like antimi
Page 525 and 526:
Penaeus monodon penaeidin-like anti
Page 527 and 528:
Penaeus monodon elongation factor m
Page 529 and 530:
Fenneropenaeus indicus anti-lipopol
Page 531 and 532:
Fenneropenaeus indicus penaeidin-li
Page 533 and 534:
Fenneropenaeus indicus beta-actin m
Page 535 and 536:
PUBLICATIONS
Page 537 and 538:
Short sequence report Molecular cha
Page 539 and 540:
Fig. 2. Multiple alignment of nucle
Page 541 and 542:
220 The phylogenetic relationships
Page 543 and 544:
Table 1 Rearing conditions and wate
Page 545 and 546:
5 ′ cttgtggttgacaatggctccg3
Page 547 and 548:
Expression of WSSV genes in the hae
Page 549 and 550:
Fig. 4. Expression profile of crust
Page 551 and 552:
S.P. Antony et al. / Immunobiology
show all

Molecular Characterization and Gene Expression Profiling ... - CUSAT

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?