Molecular Characterization and Gene Expression Profiling ... - CUSAT

Chapter 5
5.3.4. Expression profile of AMP genes in various tissues of P.
monodon in response to the administration of Candida haemulonii
yeast pre- and post-challenge WSSV
mRNAs of AMP genes could be detected in all of the target tissues at
varying levels in the animals pre- and post- challenge WSSV. The expression
profile of each AMP was found to vary for the various tissue targets (Fig.
5.18 to 5.23).
5.3.4.1. Expression profile of ALF
ALF gene transcripts could be detected at high levels in the gills,
intestine and hepatopancreas of the control group of shrimps prior to WSSV
challenge. In case of the control group of shrimps, gills and intestine
supported maximum expression of the ALF gene followed by
hepatopancreas. The ALF gene was found to be down-regulated in the gills,
intestine and hepatopancreas on CHY treatment. However, an up-regulation
of the gene could be noticed in the muscle and heart of the experimental
group of shrimps (Fig. 5. 18 A & C).
ALF gene was found to be up-regulated on WSSV challenge even in
the control group. Up-regulation of ALF could be noticed in all the tissues of
control shrimps except muscle. In CHY treated group of shrimps, a
noticeable up-regulation of the gene could be seen in case of gills, intestine
and muscle (Fig. 5. 18 B & D).
5.3.4.2. Expression profile of crustin-1
mRNA of crustin-1 gene could be detected in all of the target tissues
in the both control and CHY treated groups of animals prior to WSSV
challenge. Gill, muscle, and intestine was found to support maximum
expression of the crustin-1 gene in the control shrimps whereas in case of
CHY treated group of shrimps, gill, muscle and heart supported maximum
expression followed by intestine and hepatopancreas (Fig. 5. 19 A & C).
Molecular Characterization and Gene Expression Profiling of Antimicrobial Peptides in Penaeid Shrimps
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