Electronic Posters: Neuroimaging - ismrm
Electronic Posters: Neuroimaging - ismrm
Electronic Posters: Neuroimaging - ismrm
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Wednesday 13:30-15:30 Computer 88<br />
13:30 4510. In Vivo DTI-Derived Axial Diffusivity Correlates with Neurological Assessments in<br />
EAE-Affected Mice<br />
Joong Hee Kim 1 , Anne H. Cross 2 , Sheng-Kwei Song 3<br />
1 Radiology, Washington University , St. Louis, MO, United States; 2 Neurology, Washington University, St.<br />
Louis, MO, United States; 3 Radiology, Washington University, St. Louis, MO, United States<br />
Diffusion tensor imaging (DTI) was used to examine the spinal cords of mice with experimental autoimmune encephalomyelitis<br />
(EAE), an animal model of Multiple Sclerosis. Compared to age-matched controls, EAE-affected mice exhibited a statistically<br />
significant decrease in axial diffusivity in spinal cord white matter. The decrease of axial diffusivity was parallel to disease severity<br />
examined by clinical scoring of EAE mice. The axial diffusivity threshold analysis on EAE-affected mice enabled quantifying the<br />
extent of abnormal or damaged axons, which correlated with four independent neurological assessments.<br />
14:00 4511. Contrasting Roles for CD4 and CD8 T Cells in a Murine Model of T1 Black Hole<br />
Formation<br />
Istvan Pirko 1 , Jeremiah McDole 2 , Yi Chen 2 , Scott R. Dunn 3 , Diana M. Lindquist 3 , Aaron<br />
J. Johnson 2<br />
1 Department of Neurology, Mayo Clinic, Rochester, MN, United States; 2 Department of Neurology, University<br />
of Cincinnati, Cincinnati, OH, United States; 3 Imaging Research Center, Cincinnati Children's Hospital Medical<br />
Center, Cincinnati, OH, United States<br />
TMEV infection of mice is an accepted model of multiple sclerosis. In C57B6/J mice, the formation of T1 black holes (T1BH) is<br />
detectable in this model. In this study we confirmed that CD8 T cells are the main contributors to T1BH formation, whereas CD 4 T<br />
cells prevent T1BH formation. We also determined that the involved CD8 T cells are classic epitope specific cytotoxic T cells. T1BH<br />
formation is thought to represent neuronal/axonal damage in MS; therefore, it is plausible that CD8 T cells play an important effector<br />
role targeted at neurons and axons in MS-related neuroinflammatory diseases.<br />
14:30 4512. Monitoring Demyelination in a Cuprizone Mouse Model with Longitudinal and<br />
Quantitative MRI Measurements<br />
Jonathan D. Thiessen 1 , Yanbo Zhang 2 , Handi Zhang 2 , Lingyan Wang 2 , Richard Buist 3 ,<br />
Jiming Kong 4 , Xin-Min Li 2 , Melanie Martin 1,5<br />
1 Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada; 2 Psychiatry, University of<br />
Manitoba; 3 Radiology, University of Manitoba; 4 Human Anatomy and Cell Science, University of Manitoba;<br />
5 Physics, University of Winnipeg<br />
Magnetic resonance imaging methods capable of quantifying changes due to demyelination can improve both the diagnosis and<br />
understanding of white matter diseases such as multiple sclerosis. T2-weighted and magnetization transfer images (MTI) were<br />
acquired weekly in control (n=4) and cuprizone-fed mice (n=4) from 2 to 6 weeks of treatment. Diffusion tensor imaging, quantitative<br />
MTI, high-resolution T2-weighted imaging, and histopathology were used to analyze ex vivo tissue. All in vivo methods showed<br />
significant differences longitudinally in the corpus callosum of the cuprizone-fed mouse. All in vivo and ex vivo methods showed<br />
significant differences in the corpus callosum between groups.<br />
15:00 4513. Correlation of Fractional Anisotropy (FA) Changes in Demyelination Lesion with<br />
Its Surrounding Edema in an Experimental Model<br />
Krithika Balasubramanian 1 , Senthil S. Kumaran 1 , Uma Sharma 1 , Naranamangalam R.<br />
Jagannathan 1<br />
1 Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, Delhi, India<br />
Evaluation of sequential changes in fractional anisotropy (FA) in demyelination lesion and associated edema in an experimental rat<br />
model of demyelination was carried out at 4.7T. Results showed that both FA(lesion) and FA(edema) decreased during demyelination<br />
till day 11. Decreased FA(lesion) is attributed to the damage of myelin which progressed till day 11 while reduced FA(edema) is due<br />
to breakdown of blood-brain barrier (BBB). From day 15 remyelination set in along with repair of BBB, which led to increased<br />
FA(lesion) and FA(edema). Our study thus showed that DTI may aid in better understanding of the pathophysiology of de- and remyelination.<br />
Thursday 13:30-15:30 Computer 88<br />
13:30 4514. Study of the Pathophyisology of Demyelination in an Experimental Model:<br />
Correlation of Lesion Volume with T2, Apparent Diffusion Coefficient (ADC) and Fractional<br />
Anisotropy (FA)<br />
Krithika Balasubramanian 1 , Uma Sharma 1 , Senthil S. Kumaran 1 , Naranamangalam R.<br />
Jagannathan 1<br />
1 Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, Delhi, India<br />
Evaluation of variation in T2, ADC and FA with the lesion volume at various stages of de- and re-myelination in a demyelinating rat<br />
model was carried out at 4.7 T. During demyelination lesion size, T2 and ADC increased while FA decreased indicating loss of<br />
myelin, while these values were reversed during remyelination. A strong positive correlation was observed between lesion volume and