BULETINUL ИЗВЕСТИЯ JOURNAL

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Buletinul AŞM. Ştiinţele vieţii. Nr. 3(318) 2012 Рефераты UDC: 579.61:616+615.33:579.8 DETECTION OF ENTEROBACTERIA RESISTANCE MARKERS FOR ANTIBIOTIC. Burduniuc Olga, Cojocaru R., Balan Greta, Spînu C., Roşcin Iu. // Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii.. 2012, No 3 (318), p. 151-158. Antibiotic resistance is a complex, multifactorial problem, and is of great importance to public health, requires a complex analysis, and implementation of specifi c measures at different levels. The rapid growth of resistant strains of Enterobacteriaceae imposes the need for implementation of rapid and specifi c techniques of molecular biology. The studies emphasize that the prevalence of urinary tract infections caused by Escherichia coli, which has the resistance profi le extended-spectrum beta-lactamases type is 5.3%, and the prevalence of intestinal carriage - 15%. Detection of E.coli strains such as CTX-M-14 of the phylogenetic group B2, which have a high potential for the spread in the community, can lead to the appearance of resistance and diffi culties in the treatment of patients. Surveillance and monitoring of E.coli strains producing CTX-M plays an important role in the selection of treatment and reviewing empirical treatment protocols. 18 references, 3 fi gures. Keywords: Resistance to antibiotics - monitoring - phenotype - Molecular biology - extended spectrum beta-lactamases - CTX-M. Received January 26, 2012 UDC: 582.28:577.153.2+54.386 COORDINATIVE COMPOUNDS OF MOLYBDENUM AND VANADIUM AS POSSIBLE STIMULATORS OF EXTRACELLULAR CELLULASES BIOSYNTHESIS BY MICROMYCETES Penicillium expansum CNMN FD 05 C. Chilochi A., Tyurina J., Bologa O., Clapco S., Lablyuk S., Pasha L., Coropchanu E., Rija A. // Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii.. 2012, No 3 (318), p. 158-165. The effect of coordinative compounds of molybdenum and vanadium on the biosynthesis of cellulosolytic enzymatic complex (endoglucanases, celobiohydrolases, β-glucosidases) of the fungal strain Penicillium expansum CNMN FD 05C was investigated. It was established that complexes of molybdenum, which contain amino acids in its composition, have a neutral effect on the activity of endoglucanases, inhibit celobiohydrolases (80-90%) and stimulate the activity of β-glucosidases. Among the most effective stimulators of β-glucosidases synthesis, the complex MoO 2 (ac.ac.)Gly may be mentioned, that increases the enzymatic activity by 47.8- 67.0%. This complex can be used to obtain an enzymatic preparation with a high content of β-glucosidases. The metal complex (NH 4 ) 2 VO 3 Gly stimulates the activity of enzymes of the cellulosolytic complex of the fungi Penicillium expansum CNMN FD 05C, increasing the endogluconasic activity by 45%, cellobiohydrolasic by 32% and β-glucosidasic by 40%. 16 references, 3 tables, 3 fi gures. Key words: Penicillium expansum - metal complex – endoglucanases – cellobiohydrolases - β-glucosidases Received October 22, 2012 UDC: 576.8 STANDARTIZATION OF THE CONSERVATION METHOD FOR BACTERIA STRAINS FROM THE NATIONAL COLLECTION OF NONPATHOGENIC MICROORGANISMS. II. STANDARTIZATION OF THE CONSERVATION METHOD FOR BACILLUS STRAINS FROM THE NATIONAL COLLECTION OF NONPATHOGENIC MICROORGANISMS. Tolochikina S., Slanina V., Boortseva S., Postolaky O., Syrbu T., Lupashku L., Stepanov V. // Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii.. 2012, No 3 (318), p. 165-172. 204
Buletinul AŞM. Ştiinţele vieţii. Nr. 3(318) 2012 Рефераты A nutrient medium for the cultivation of bacteria genus Bacillus (deposited in NCNM) before the freeze-drying that ensures a maximum preservation of viability and stability of the characteristics was selected. The protective properties of a range of media for lyophilisation of microorganisms were studied. The optimum viability was achieved by using skim milk + 5% sucrose and 5% lactose. is the Distilled water is the best rehydrator in the reactivation process 19 references, 6 tables. Keywords: freeze-drying - nutrient medium - protective medium - viability Received April 19, 2012 UDC: 574 : 504 (478-25) THE PROBLEMS OF SUSTAINABLE DEVELOPMENT OF THE CHISINAU URBAN ECOSYSTEM. Bulimaga C. // Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii.. 2012, No 3 (318), p. 172-181. The main problems, which refl ect the ecological state of the Chisinau urban ecosystem have been distiguished. The principles and objectives of the social-economic development were estabilished. The ways for sustainable development of the urban ecosytem were proposed, as a basis for elaboration of the Local Action Plan regarding the Environment Control of the Chisinau municipality. 16 references Key words: urban areas - environmental capacity - fl exibility - reversibility - social and cultural diversity Received February 07, 2012 205
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