Protocols and Applications Guide (US Letter Size) - Promega
Protocols and Applications Guide (US Letter Size) - Promega
Protocols and Applications Guide (US Letter Size) - Promega
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||||||||| 9DNA Purification<br />
The Maxwell® 16 Instrument is easy to set up—just unpack<br />
<strong>and</strong> begin to use. No training or external computer<br />
required, so the instrument is ready for immediate use.<br />
Optimized automated methods are preloaded, the prefilled<br />
reagent cartridges are snapped into place, your sample is<br />
added <strong>and</strong> you press "Start" to begin the appropriate<br />
method.<br />
For challenging sample types or more control over the DNA<br />
purification application, the Maxwell® 16 Flexi Method<br />
Firmware (Cat.# AS6411) allows you to create your own<br />
program by optimizing the lysis, binding, drying, elution<br />
<strong>and</strong> paramagnetic particle capture parameters. Parameters<br />
are entered using the on-screen prompts on the<br />
Maxwell® 16 Instrument. The Maxwell® 16 Flexi Method<br />
Firmware is available as an option for new Maxwell® 16<br />
Instruments <strong>and</strong> can be installed on existing instruments.<br />
In addition to kits <strong>and</strong> methods developed by <strong>Promega</strong><br />
[e.g., the Maxwell® 16 Cell LEV DNA Purification Kit (Cat.#<br />
AS1140) <strong>and</strong> the Maxwell® 16 FFPE Tissue LEV DNA<br />
Purification Kit (Cat.# AS1130)], customers <strong>and</strong> <strong>Promega</strong><br />
applications scientists are continuously developing new<br />
applications for the Maxwell® 16 System. To facilitate<br />
sharing information about samples tested by both <strong>Promega</strong><br />
scientists <strong>and</strong> Maxwell® 16 users throughout the world,<br />
the Maxwell® 16 <strong>Applications</strong> Database<br />
(www.promega.com/maxwell16/applications) is available.<br />
This online resource allows you to search by keyword or<br />
browse through folders organized by sample type <strong>and</strong><br />
applications, <strong>and</strong> contains information on samples<br />
processed, yields achieved <strong>and</strong> downstream applications<br />
tested. You can submit entries to the database using the<br />
provided submission form (www.promega.com<br />
/maxwell16/applications/submission.asp). Recent<br />
applications include RNA <strong>and</strong> DNA from Spirulina<br />
(blue-green algae) <strong>and</strong> total RNA from Gram Negative<br />
bacterial culture.<br />
Automation eliminates the h<strong>and</strong>s-on time <strong>and</strong> labor of<br />
manual purification, giving you the time to focus on your<br />
research. In addition, the Maxwell® 16 Instrument design,<br />
optimized reagents <strong>and</strong> automated methods provide<br />
consistent yield <strong>and</strong> purity for your downstream<br />
applications. The instrument is benchtop compact <strong>and</strong> can<br />
purify from many sample types (Table 9.4). Future<br />
automated method updates can be added through the<br />
instrument communications port.<br />
<strong>Protocols</strong> & <strong>Applications</strong> <strong>Guide</strong><br />
www.promega.com<br />
rev. 3/09<br />
Table 9.4. DNA yield from various sample types after<br />
purification using the Maxwell® 16 Instrument <strong>and</strong> DNA<br />
Purification Kits.<br />
Sample<br />
Sample Type<br />
Whole blood<br />
Whole blood<br />
Mouse tail<br />
Animal tissue<br />
Tissue culture<br />
cells<br />
Gram–<br />
bacteria<br />
Gram+<br />
bacteria1 Plant leaf<br />
(tomato) 1<br />
Drosophila<br />
melanogaster<br />
Drosophila<br />
melanogaster<br />
Caenorhabditis<br />
elegans<br />
Danio reri<br />
Saccharomyces<br />
cerevisiae 2<br />
Arabidopsis<br />
thaliana<br />
1 With optional pretreatment.<br />
<strong>Size</strong><br />
200µl<br />
400µl<br />
1.2cm<br />
20–25mg<br />
5 × 106<br />
2 × 109<br />
2 × 109<br />
25mg<br />
1 fly<br />
5 flies<br />
~50,000<br />
worms<br />
50mg<br />
1 colony<br />
(3mm)<br />
1 leaf<br />
Yield<br />
4–9µg (>3pg/white<br />
blood cell)<br />
8–15µg (>3pg/white<br />
blood cell)<br />
≥20µg<br />
60–100µg (mouse<br />
liver)<br />
15–20µg (HeLa)<br />
25–30µg (E. coli BL21)<br />
15–25µg (B. cereus)<br />
9–13µg<br />
0.32µg<br />
1.52µg<br />
0.08µg<br />
24.8µg<br />
0.72µg<br />
0.13µg<br />
2 Includes 3 hour digestion with lyticase prior to DNA isolation.<br />
Additional Resources for Maxwell® 16 System<br />
Technical Bulletins <strong>and</strong> Manuals<br />
TM295 Maxwell® 16 Instrument Operating Manual<br />
(www.promega.com<br />
/tbs/tm295/tm295.html)<br />
TM284 Maxwell® 16 DNA Purification Kits<br />
Technical Manual<br />
(www.promega.com<br />
/tbs/tm284/tm284.html)<br />
TM309 Maxwell® 16 Mouse Tail DNA Purification<br />
Kit Technical Manual<br />
(www.promega.com<br />
/tbs/tm309/tm309.html)<br />
TB383 Maxwell® 16 Cell LEV DNA Purification Kit<br />
Technical Bulletin<br />
(www.promega.com/tbs/tb383/tb383.html)<br />
TB382 Maxwell® 16 FFPE Tissue LEV DNA<br />
Purification Kit Technical Bulletin<br />
(www.promega.com/tbs/tb382/tb382.html)<br />
PROTOCOLS & APPLICATIONS GUIDE 9-23
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