Protocols and Applications Guide (US Letter Size) - Promega

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||||||||||||| 13Cloning VII. References Anderson, S. et al. (1980) A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2. Nucleic Acids Res. 8, 1731–43. Burd, J.F. and Wells, R.D. (1974) Synthesis and characterization of the duplex block polymer d(C15A15)-d(T15G15). J. Biol. Chem. 249, 7094–101. Challberg, M.D. and Englund, P.T. (1980) Specific labeling of 3' termini with T4 DNA polymerase. Methods Enzymol. 65, 39–43. Clark, J.M. (1988) Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 16, 9677–86. D’Avino, P.P. et al. (2004) Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac. J. Cell Biol. 166, 61–71. Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY. Grosveld, F.G. et al. (1981) Isolation of β-globin-related genes from a human cosmid library. Gene 13, 227–37. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109. Knoche, K. and Kephart, D. (1999) Cloning blunt-end Pfu DNA polymerase-generated PCR fragments into pGEM®-T Vector Systems. Promega Notes 71, 10–4. Le Gall, S.M. et al. (2003) Regulated cell surface pro-EGF ectodomain shedding is a zinc metalloprotease-dependent process. J. Biol. Chem. 278, 45255–68. Meyerowitz, E.M. et al. (1980) A new high-capacity cosmid vector and its use. Gene 11, 271–82. Mezei, L.M. and Storts, D.R. (1994) Purification of PCR products. In: PCR Technology: Current Innovations, Griffin, H.G. and Griffin, A.M., eds., CRC Press, Boca Raton, FL, 21. Newton, C.R. and Graham, A. (1994) In: PCR, BIOS Scientific Publishers, Ltd., Oxford, UK, 13. Robles, J. and Doers, M. (1994) pGEM®-T Vector Systems troubleshooting guide. Promega Notes 45, 19–20. Sakakida, Y. et al. (2005) Importin α/β mediates nuclear transport of a mammalian circadian clock component, mCRY2, together with mPER2, through a bipartite nuclear localization signal. J. Biol. Chem. 280, 13272–8. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Seeburg, P.H. et al. (1977) Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature 270, 486–94. Studier, F.W. and Moffat, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113–30. Ullrich, A. et al. (1977) Rat insulin genes: Construction of plasmids containing the coding sequences. Science 196, 1313–9. Protocols & Applications Guide www.promega.com rev. 3/09 Yanisch-Perron, C. et al. (1985) Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103–19. Yoshimura, H. et al. (1986) Biological characteristics of palindromic DNA (II). J. Gen. Appl. Microbiol. 32, 393–404. 4-CORE, Flexi, GoTaq, HaloTag, MagneSil, pGEM and Wizard are registered trademarks of Promega Corporation. AccessQuick, AgarACE, GeneEditor, LigaFast, MULTI-CORE, PinPoint, PureYield, Select96, pTARGET, SILVER SEQUENCE and SoftLink are trademarks of Promega Corporation. Bacto is a registered trademark of Difco Laboratories, Detroit, Michigan. Ficoll is a registered trademark of GE Healthcare Bio-sciences. Gateway is a registered trademark of Invitrogen Corporation. Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information. All prices and specifications are subject to change without prior notice. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. © 2004–2009 Promega Corporation. All Rights Reserved. PROTOCOLS & APPLICATIONS GUIDE 13-23
|||||||||||||| 14DNA-Based Human Identification CONTENTS I. Introduction 1 A. Variable Number Tandem Repeats and Restriction Fragment Length Polymorphisms 1 B. Short Tandem Repeats 1 C. Single Nucleotide Polymorphisms 1 D. Mitochondrial DNA Analysis 2 II. DNA Purification 2 A. DNA IQ System—An Example of a Magnetic Resin 3 III. Differential Extraction 4 IV. DNA Quantitation 4 V. STR Analysis 6 A. STR Amplification and Detection 6 B. Data Analysis 6 C. Statistical Analysis 7 VI. Automation 9 A. Maxwell® 16 Instrument 9 B. High-Throughput Automation Workstations 9 C. Automated DNA Purification 10 D. Automated Differential Extraction 11 E. Automated DNA Normalization and PCR Setup 12 VII. References 12 Protocols & Applications Guide www.promega.com rev. 6/09 PROTOCOLS & APPLICATIONS GUIDE
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CONTENTS I. Nucleic Acid Amplificat
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||| 3Apoptosis I. Introduction A. C
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||||||| 7Cell Signaling CONTENTS I.
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