Protocols and Applications Guide (US Letter Size) - Promega
Protocols and Applications Guide (US Letter Size) - Promega
Protocols and Applications Guide (US Letter Size) - Promega
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|||||||||| 10Cell Imaging<br />
Catalog Number<br />
Cat.# G1641<br />
Known Species<br />
Cross-Reactivity<br />
Human, mouse, rat, rabbit2 <strong>and</strong> quail2 Cat.# G1631<br />
Rat, mouse, human <strong>and</strong> cow 2<br />
Cat.# G2791<br />
Human, mouse, rat <strong>and</strong><br />
Rhesus monkey<br />
Cat.# G1132; Cat.# G1131<br />
Human, mouse, guinea pig,<br />
rat, rabbit, goat, sheep, cow,<br />
pig, horse, cat2 Cat.# G1651<br />
Human, mouse, rat <strong>and</strong> cat 2<br />
Cat.# G1221<br />
Human, mouse, rat <strong>and</strong> cat 2<br />
Cat.# G3231<br />
Human, mouse, rat <strong>and</strong><br />
chicken (species crossreactivity<br />
due to high<br />
conservation of cytoplasmic<br />
domain)<br />
Table 10.1. Additional Antibodies Available From <strong>Promega</strong>.<br />
Application <strong>and</strong><br />
Recommended Dilution1 Antibody<br />
Species (subclass)<br />
Anti-Human BNDF Chicken (IgY)<br />
Western 1µg/ml; ELISA<br />
pAb<br />
1µg/ml; ICC 1–10µg/ml; IHC<br />
10–15µg/ml; BN 10µg/ml<br />
Anti-Rat CNTF pAb Chicken (IgY)<br />
Western 1µg/ml; ELISA<br />
1µg/ml; ICC 1–10µg/ml<br />
Western 1µg/ml; ELISA<br />
1µg/ml; ICC2 Anti-Human GDNF Chicken (IgY)<br />
pAb<br />
Anti-NGF mAb Rat (IgG)<br />
Western 1µg/ml; ELISA<br />
10.5µg/ml; ICC, IHC<br />
0.5–1.0µg/ml; BN not<br />
recommended<br />
Western 1µg/ml; ELISA<br />
1µg/ml; ICC 1–10µg/ml; IHC2; Anti-Human NT-3 pAb Chicken (IgY)<br />
BN 1–10µg/ml<br />
Western 1:1,000 dilution;<br />
ELISA not recommended; IHC<br />
1:50 diltuion; BN2 Anti-TBFβ1 pAb Rabbit (IgG)<br />
Anti-Human p75 pAb Rabbit (IgG)<br />
Western not recommended; IP<br />
1–10µg/ml; ICC, IHC<br />
1–10µg/ml; BN 1–10µg/ml<br />
<strong>Protocols</strong> & <strong>Applications</strong> <strong>Guide</strong><br />
www.promega.com<br />
rev. 3/09<br />
Cat.# G1561<br />
Human, rat <strong>and</strong> mouse 2<br />
Chicken (IgY)<br />
Anti-TrkB In pAb<br />
Cat.# G1581<br />
TrkA, TrkB <strong>and</strong> TrkC due to<br />
the highly conserved<br />
ALAQAPPVYLDVL sequence<br />
of human, rat <strong>and</strong> mouse<br />
Human <strong>and</strong> nonhuman<br />
primates; marker for mast cells<br />
ICC, IHC 1–10µg/ml; BN not<br />
recommended<br />
Western 1µg/ml; ELISA<br />
1µg/ml; ICC, IHC 1–10µg/ml;<br />
IP 0.5µg/ml; BN not<br />
recommended<br />
Western 1:10,000 dilution;<br />
ELISA 1:2,000–1:5,000 dilution;<br />
ICC, IHC 1:1,000 dilution<br />
Chicken (IgY)<br />
Anti-Pan Trk pAb<br />
Cat.# G3361<br />
Mouse<br />
Anti-Human Tryptase<br />
mAb Biotin<br />
1 Investigators should optimize contentrations for their specific applications <strong>and</strong> conditions.<br />
2 This information was not generated by <strong>Promega</strong> scientists but has been published in the scientific literature. Please contact <strong>Promega</strong> Technical Services or refer to the <strong>Promega</strong><br />
Antibody Assistant (www.promega.com/techserv/tools/abasst/) for additional information.<br />
3<br />
Key: ICC = immunocytochemistry; IHC = immunohistochemistry; BN = biological neutralization; IP = immunoprecipitation<br />
PROTOCOLS & APPLICATIONS GUIDE 10-18
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