Protocols and Applications Guide (US Letter Size) - Promega

||||||||||| 11Protein Purification and Analysis
Prepare Samples
1. Add an equal volume of loading 2X buffer to the
sample.
2. Incubate the sample at 95°C for 2–5 minutes, mix by
vortexing and load onto the gel.
Optional
1. If the sample is very dilute or contains salts that may
interfere with gel analysis, add TCA to a final
concentration of 10% (w/v).
2. Place the sample on ice for 5 minutes, centrifuge at 4°C
for 2 minutes at 12,000 × g in a microcentrifuge and
discard the supernatant.
3. Wash the protein pellet with ice-cold acetone, and
resuspend it in a suitable volume (generally