Protocols and Applications Guide (US Letter Size) - Promega

||||||||||| 11Protein Purification and Analysis
polyhistidine-tag
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Lyse and Bind
Native Conditions Denaturing Conditions
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Cells
HisLink
Ni-Particle
Wash
Elute
Pure Native Protein Pure Denatured Protein
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Transfer to Filter Plate
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polyhistidine-tag
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Figure 11.4. A schematic representation of the HisLink 96 Protein Purification protocol.
Protocols & Applications Guide
www.promega.com
rev. 3/09
4867MB
PROTOCOLS & APPLICATIONS GUIDE 11-8