Protocols and Applications Guide (US Letter Size) - Promega

|||||||||| 10Cell Imaging
Anti-VAChT pAb
The purified Anti-VAChT (Vesicular Acetylcholine
Transporter) pAb (Cat.# G4481) is raised in goats against
a peptide (CSPPGPFDGCEDDYNYYSRS) corresponding
to amino acids 511–530 of the carboxy terminus of the
cloned rat VAChT. It is a novel tool to identify functional
cholinergic neurons in the central and peripheral nervous
system where the antibody stains fibers and neuronal cell
bodies. This antibody has application for the study of the
pathophysiology of neurodegenerative diseases involving
the cholinergic system and for mapping cholinergic neurons
in the nervous system.
• Immunogen: Carboxy-terminal peptide sequence
511–530 corresponding to cloned rat VAChT protein.
• Antibody Form: Purified goat polyclonal IgG; 0.5mg/ml
in PBS containing no preservatives.
• Specificity: Cross-reacts with VAChT in rat and mouse,
but not in human, guinea pig, rabbit or cat.
• Suggested Dilution: 1:500 for immunohistochemistry.
Additional Resources for Anti-VAChT Antibody
Promega Publications
NN010 Immunostaining with Promega Reagents:
Anti-VAChT pAb (514kb pdf)
(www.promega.com
/nnotes/nn303/303_12.pdf)
Online Tools
Antibody Assistant (www.promega.com
/techserv/tools/abasst/antibodypages/antivachtpab.htm)
D. Other Antibodies
Promega offers a variety of antibodies against growth
factors, neurotrophic factor receptors and other molecules.
Usage information for these antibodies is summarized in
Table 10.1. For additional information on these antibodies,
please visit the Antibody Assistant (www.promega.com
/techserv/tools/abasst/).
V. References
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scans. Nature 435, 254–5.
Eisenstein, M. (2006) Helping cells to tell a colorful tale. Nature
Methods 3 647–55.
Lang, C. et al. (2006) HaloTag®: A new versatile reporter gene
system in plant cells. J. Exp. Bot 57 2985–92.
Los, G.V. et al. (2005) HaloTag Interchangeable Labeling
Technology for cell imaging and protein capture. Cell Notes 11,
2–6.
McKenna, N.M. and Wang, Y-l. (1989) Culturing cells on the
microscope stage. Methods in Cell Biol. 29, 1–12. San Diego, CA:
Academic Press.
Owen, R.M. et al. (2002) Synthesis and applications of end-labeled
neoglycopolymers. Org. Lett. 4 2293–6.
Shinohara, H. and Matsubayahsi, Y. (2007) Functional
immobilization of plant receptor-like kinase onto microbeads
Protocols & Applications Guide
www.promega.com
rev. 3/09
toward receptor array construction and receptor-based ligand
fishing. Plant J. 52 175–84.
Stephens, D.J. and Allan, V.J. (2003) Light microscopy techniques
for live cell imaging. Science 300, 82–86.
Sullivan, K.F. and Kay, S.A. (1999) Preface. Methods in Cell Biol. 58,
xv–xvi.
Wang, Y-l. (1989) Fluorescent analog cytochemistry: Tracing
functional protein components in living cells. Methods in Cell Biol.
29, 1–12.
Zhang, Y. et al. (2006) HaloTag® protein-mediated site-specific
conjugation of bioluminescent proteins to quantum dots. Angew.
Chem. Int. Ed. Engl. 45 4936–40.
Anti-ACTIVE, CellTiter-Glo, HaloTag and Monster Green are registered
trademarks of Promega Corporation. CaspACE, MaxxPure and TransFast
are trademarks of Promega Corporation.
Alexa Fluor and Oregon Green are registered trademarks of Molecular
Probes, Inc. Axiovert is a registered trademark of Carl Zeiss, Inc. Cy is a
registered trademark of Amersham Biosciences, Ltd. FACS is a registered
trademark of Becton, Dickinson and Company. Hemo-De is a registered
trademark of Scientific Safety Solvents. NANOpure is a registered trademark
of Barnstead/Thermolyne Corporation. Triton is a registered trademark of
Union Carbide Chemicals & Plastics Technology Corporation. Tween is a
registered trademark of ICI Americas, Inc. VECTASHIELD is a registered
trademark of Vector Laboratories, Inc.
Products may be covered by pending or issued patents or may have certain
limitations. Please visit our Web site for more information.
All prices and specifications are subject to change without prior notice.
Product claims are subject to change. Please contact Promega Technical
Services or access the Promega online catalog for the most up-to-date
information on Promega products.
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PROTOCOLS & APPLICATIONS GUIDE 10-17