Appendices 5-13 - Nautilus Cares - Nautilus Minerals

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CSIRO Land and Water Page 1 of 3 Centre for Environmental Contaminants Research (CECR) Lucas Heights Science and Technology Centre New Illawarra Road, Lucas Heights NSW 2234 Private Mailbag 7, Bangor NSW 2234 Australia Telephone: +61 2 9710 6777 Facsimile: +61 2 9710 6800 www.csiro.au ABN 41 687 119 230 Juvenile Amphipod Whole Sediment Test Report MpL 020508 Client: Coffey Natural Systems Project: Solwara Test Performed: 10-day survival whole-sediment toxicity test using the juvenile amphipod Melita plumulosa Test Method This acute test measures the survival of juvenile amphipods (Melita plumulosa) following exposure to test sediments over a 10-day period. This test was carried out according to the protocols described in Simpson et al. (2005) and King et al. (2006). Sediments were homogenised immediately prior to being added to test beakers (38- 40 g sediment per beaker, 5 replicates per sediment). Filtered seawater (30‰) was added, and each beaker was incubated at 21 o C with aeration overnight to allow sediments to settle. The following day, overlying water was replaced and 15 amphipods (7-14 days old) were randomly assigned to each beaker. Amphipods used in the tests were obtained from laboratory cultures. Treatments were fed 0.063 mg Sera micron fish food/juvenile every 3 days. Overlying water temperature, pH, salinity and dissolved oxygen were measured throughout the 10-day test. The number of surviving amphipods in each beaker was recorded after 10 days, and sediments were also stained to ensure full recovery of all surviving amphipods. Results are expressed as a percentage of the survival in the diluent control sediment. Also on day 10, three sub-samples were taken from each dilution, filtered (0.45 !m), acidifed (2% Tracepure HNO3) and analysied by ICP-AES for dissolved metals. A reference toxicant test (96-h water exposure to copper), using the same batch of juveniles, was carried out concurrently with the whole sediment tests. The median lethal concentration (LC50) and the 95% confidence limits were estimated by Maximum Likelihood Regression using Probit Analysis with Abbotts correction (Toxcalc, Version 5.0.23C). Sample Preparation Samples Collected: Samples Received: <strong>13</strong>/03/2008 Test Initiated: 22/04/2008 CSIRO Sample No. Sample Name Sample Description WQE08070 A10988 Dry silty sand The sample was stored at 4°C and homogenised immediately prior to use in the test. Dilutions of the Solwara sediment were prepared by combining the dry 100% Solwara sediment (A10988) and the clean dry diluent sediment (sand) to achieve the required percentage by weight. Results Physico-chemical properties Particle Size Distribution of the Solwara Sediment and the Dilutions Test Material Size Fractionation (%) < 63 !m 63-180 !m >180 !m Diluent sediment 0 1 99 1% Solwara Sediment 0 1 99 5% Solwara Sediment 1 1 98 10% Solwara Sediment 2 2 96 33% Solwara Sediment 5 5 90 100% Solwara Sediment 14 14 72 Comments A u s t r a l i a n S c i e n c e , A u s t r a l i a ’ s F u t u r e
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Page 4 and 5: Project director David Gwyther Proj
Page 7: Appendix 8 Juvenile Amphipod Whole
Page 11 and 12: Page 3 of 3 Spadaro, D.A., Micevska
Page 13 and 14: Centre for Environmental Contaminan
Page 15: Centre for Environmental Contaminan
Page 19 and 20: Tropical Futures: Mineral Program i
Page 21 and 22: 1. Introduction The initial offshor
Page 23 and 24: and minimize air oxidation. However
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Page 27 and 28: Table 2: pH, redox (Eh), conductivi
Page 29 and 30: 3..2 Characterisation of 0.45 !m fi
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Page 35 and 36: depending on the actual chemical fo
Page 37 and 38: Table 6: Major ions in 0.45 !m filt
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Page 42 and 43: Appendix 1: Phase 1: Metal concentr
Page 44 and 45: Appendix 3: Phase 1: Quality contro
Page 46 and 47: Appendix 5: Phase 2: Metal concentr
Page 48 and 49: Appendix 7: Phase 2: Net metals rel
Page 51: Appendix 10 Biomass, Biodiversity a
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Appendix 11 Modelling the Dispersio
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Document control form Document draf
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1 EXECUTIVE SUMMARY This report qua
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2.2 Bathymetry High resolution digi
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Nautilus took samples of the sedime
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Prior to mining, the surface uncons
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Figure 3.1. Sediment bottom thickne
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Figure 3.3a. Sediment bottom thickn
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Figure 3.3c. Sediment bottom thickn
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Figure 3.3e. Sediment bottom thickn
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Appendix 12 Modelling the Dispersio
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Document control form Document draf
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1 EXECUTIVE SUMMARY The following r
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2 MODELLING METHODOLOGY AND DATA 2.
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Stochastic modelling was carried ou
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Figure 2.2. Temperature profile at
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Figure 2.3. Location Map showing th
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For these scenarios, the dual disch
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3.3 Affected areas and extents In T
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3.5 Sedimentation Thickness The 1:5
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Figure 3.3. The expected (or averag
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Figure 3.5 Bottom sedimentation pat
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French, D., Schuttenberg, H., Isaji
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Appendix 13 Prediction of Underwate
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Abstract This report presents the r
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List of Figures Figure 1. Geographi
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Figure 1. Geographical location of
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Figure 2. The corresponding broadba
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corresponding to the eight directio
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Depth (m) -1300 -1400 -1500 -1600 -
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3 Results Plots of the received lev
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Figure 8. Plots of predicted receiv
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Figure 10. Scatter plot of received
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* Noise levels attenuate rapidly wi
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4.2! Potential impacts on marine ma
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conditions (noting that for a signa
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slowly, or moves independently of a
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The noise is likely to be audible t
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Watkins, W.A. (1986). Whale reactio
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The Potential for Natural Disasters
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the coast of the Bismarck Sea. Thes
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A tsunami can be generated by any d
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Environmental Impact Statement Solw
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