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Computational tools and Interoperability in Comparative ... - CBS

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rRNA operons <strong>and</strong> promoter analysis<br />

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Figure 3.4: Logo plots show<strong>in</strong>g the <strong>in</strong>itial weight matrices used for search<strong>in</strong>g E. coli <strong>and</strong> Shigella<br />

genomes: –10 hexamer (a), –35 hexamer (b), UP element (c), <strong>and</strong> FIS b<strong>in</strong>d<strong>in</strong>g motif (d).<br />

The 16S rRNA genes of all E. coli <strong>and</strong> Shigella genomes were annotated us<strong>in</strong>g RNAmmer.<br />

For the list of genomes, see table 3.1. All 16S rRNA genes were aligned us<strong>in</strong>g clustalw<br />

(Thompson et al., 1994) <strong>and</strong> a neighbor-jo<strong>in</strong><strong>in</strong>g tree was constructed (see figure 3.5). The<br />

figure shows additional Salmonella <strong>and</strong> Yers<strong>in</strong>ia genomes for comparison.<br />

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