bioreactor studies of heterologous protein production by ...

From a semilogarithmic plot @gm 4. M), values of at different dilution rates
can be obtained by linear regression. From Eq. (1 1) and (17), we have C, = -k, ,
C, = kt and b = k, respectively. Using Eq. (12). (13) and (14). the specific growth rate of
plasmid-bearing tells ( p' = Ct + b + D = D ). the speafic growth rate of phnid-fiee ceils
(p- =Cl +C,+D=D). probability of plasmid loss (p =b/(CÎ+b+D) =kf/D) at
diffennt diïution rates were estimated. The values of p+, p-, p, and kt at various dilution
rates are compiled in Table 4.3. The dB/dt vernis B plot for each dilution rate is
iUustrated in Figure 4.16.
Table 4.3. Estimated Values of Instabüity Parsmeters Dunng Continuous Culture
of Recombinant S. cerevkiae (ATCC 20690) in Airlift Bioreactor.