bioreactor studies of heterologous protein production by ...

B
Table 4.5. Metabolic Pathway Analysis during Continuous Cuiture in ALR
Diiution Rate D (lil)
Total Cd Masr X (g/L)
Ethanol Concentration E (%L)
2.2%7 4.8626 7.0343
1 1 1
I
Cell Mas Contributed from 0.5659 12405 1.7945
Glueose Fermentation XI (fi) =
1 Ceu Mas Contrîbuted fmm Glucose ( 6.5021 ( 4.2058 1 19904 1
Oxidation Xr (fi) = X - XI
0.05
%O680
Fraction of Ce& Produced in Glume
Fermentation (X1/X)
O.OS00 0.2278 0.4741
I
Fraction of Cells Produceci in Glume 1 Oxidation (X6)
09200 1 0m8 1 1
4.3.5. Cornparison between ALR and STR Bioreactors
Continuous culture experiments using nonselective medium were also performed in the
STR bioreactor. Cornparisons of the enzyme. tell and ethanol concentrations between the
ALR and STR bioreactors in continuous culture (diiution rate = 0.10 h") are pnsented in
Figure 4.22 (ushg original strain) and Figare 4.23 (using se1ected strain). Figures 4.22
and 4.23 show that the enzyme concentrations were higher m the ALR bioreactor even
though the œiï concentrations were lower. Thenfore, the eqme yield or speafic
enzyme activity was lower in the STR bioreactor, which is consistent with the nsults
obtained in batch fermentation as discussed in Section 4.2.3. It û also intereshg to note
0.10
5.4463
0.20
3.7849
1