bioreactor studies of heterologous protein production by ...

53. Modei Simulation in Batch CuIture
5.3.1 System Equations for Batch Culture
When the above model is appiied to a batch fermentation process, the following dymnic
equations are generated:
Glucose (G):
Total Ceii Mass (X):
rProtein (P):
Fraction of PBC (F): F=- " ~100
X
(5.14
(S. 12)
The batch culture model requires 15 equations and 18 parameters. Using the
parameter estimates h m experimental data, the model can be solved simultaneously for
six variables (G, X, X+, E, P and F) by C cornputer programming.
5.32 Parameter Estimation
The parameter values used for model simdation are summhd in Table 1. The values of
dl these parameters are within the mnge of litmm values. Of the 18 parameters, 14
were estimated h m the experimentai resuia, 4 were derived hm literature. AU