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bioreactor studies of heterologous protein production by ...

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I*Plasrnid-bearing ceU concentration dynamics *I<br />

tloat Fxp(float g. float q, Boat e. float xp, float t)<br />

float fxp:<br />

&p=( 1 .O-ppl)*Miu(g, q, e. t)*xp;<br />

retum fxp;<br />

1<br />

/* Ethanol concentration dynamics */<br />

tloat Fe(floar g. noat q, float e. float x, flou t)<br />

{<br />

float fe;<br />

fe=Yex+Miu I(g, q. t)*x - Miu3(qT e)*xlYxe;<br />

return fe;<br />

1<br />

1* Glucoamylase concentration dynmics */<br />

tloat Fp(îloat _o. tloat q. float r. float xp. float t)<br />

{<br />

float fp;<br />

fp=Ypx2*Miu2(g, q. t)*xp + Ypx3*Miu3(q, e)*xp;<br />

remm fp:<br />

1<br />

IL Specific p wth ate for glucose lermenrauon V<br />

float Miu 1 (float g, float q, float t)<br />

{<br />

f oat miu I ;<br />

miu 1 =L(t)*M I *g/(K I Y)*( 1 .O+ka*q)l(kb+ka*q);<br />

return rniu 1 ;<br />

1

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