bioreactor studies of heterologous protein production by ...

Table 4.4. Cornparison between Values of M d and Predicted kr.
4.3.3.6. Conclusions
A simple khetic model was derived for expresshg piaand stabiltty in a recombinant yeast
in continuous culture. The plasmid loss foliowed Wt-order kinetics, which agreed weli
with the experimental resuits. A novel method was developed for estimation of the
specifk growth rates of plasmid-bearing and plasmid-fiee cek, and the probability of
plasmid loss. In the preant case, the intrinsic probability of pIasmid los remained
constant and independent of the dilution rate. and the specinc growth rates of the phsrnid-
bedg and plasmid-frce populations were identical. However, the model cm be applied
to situations when differences exist in the above parameter values.