bioreactor studies of heterologous protein production by ...

5.1 Introduction
CHAPTER 5
MODELING OF RECOMBINANT S. CEREVISIAE
Many genetic and environmental factors must be considend in developing a recombinant
cell bioprocess. Quantitative rnodeling of nxombinant cells is very useful for organizing
the iiterature and experirnental information, understanding the nature of recombinants. and
design, scale-up, operation, control and optimization of recombinant bioprocesses.
In this chapter. a metabolic mode1 describing ceii growth. substrate consumption,
ncombinant protein production and plasmid segregation of recombinant yeast is proposd
by utiüzing the information obtained in this work and the avaiiable knowledge in the
üteranue. Although the model's simplinty and feasibility for engineering applications are
emphasized, the key metabolic features d yeast are still incorporatecl into the modeL
Experimental results in both batch and continuous fermentations of the recombinant S.
cerevisiae C468IpGAC9. are w d to evaluate the accuracy and feasibility of the model m
descnbing the obselved phenomena To test the generality of the model, verification k
ais0 performed with sets of experimental data hm independent studies for other
recombinant yeast saains.