in vitro culture and isoenzyme analysis of giardia lamblia

Table 5.2. Sensitivity to Ciprofloxacin of Giardia reference strains WB and H7: ~ummary
of growth rank of the culture tubes containing different Ciprofloxacin concentrations after
20 and 48 hours incubation.
5 2 1 3 2
2,5 2 1 3 2
1,25 2 1 4 2
0,625 2 1 3 2
0,3125 2 1 3 2
° (control) 2 1 3 2
5.3.2 Cultivation of In Vitro Excysted Trophozoites.
Table 5.3 summarises the results of all culture initiates derived from in vitro
excystations.
The behaviour of in vitro excysted trophozoites was not consistent in culture.
Some survived for up to six hours, rounded up and died. Others survived for 12,
24 or 72 hours or as long as 9 days in culture but gradually died. The majority of
these never adhered to the culture tube surface but swam around vigorously and
later became sluggish. A few attached to the glass but remained as single cells at
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