in vitro culture and isoenzyme analysis of giardia lamblia
in vitro culture and isoenzyme analysis of giardia lamblia
in vitro culture and isoenzyme analysis of giardia lamblia
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For GPI activity, different sub<strong>culture</strong>s <strong>of</strong> the WB isolate showed consistent b<strong>and</strong>s<br />
(Plate7.2). Poor separation <strong>of</strong> E. histolytica (result<strong>in</strong>g from a shorter run time) was<br />
observed. The Giardia enzymes migrated cathodally at pH 7,0. E. histolytica<br />
enzyme migrated anodally. Attempts to alter the direction <strong>of</strong> migration by<br />
chang<strong>in</strong>g the pH <strong>of</strong> the bridge buffer to 8.6 were unsuccessful as no b<strong>and</strong>s were<br />
obta<strong>in</strong>ed at this pH. S<strong>in</strong>gle b<strong>and</strong>s were displayed by all isolates. There were 2<br />
patterns where<strong>in</strong> the reference stra<strong>in</strong>-WB <strong>and</strong> local isolates SA6, SA24 <strong>and</strong><br />
SA305 showed a shorter migration distance than the reference stra<strong>in</strong> H7 <strong>and</strong> local<br />
isolates SA7, SA18 <strong>and</strong> SA29 (Plate 7.3)<br />
Plate 7.3. Glucosephosphate isomerase b<strong>and</strong>s.<br />
Lane1. Un<strong>in</strong>oculated TVI medium. Lane 2. E. histolityca control. Lanes 3-11<br />
G.<strong>lamblia</strong> trophozoites H7, WB, SA6, SA7, SA18, SA24, SA29a, SA29b <strong>and</strong><br />
SA305. (SA29a =27 sub<strong>culture</strong>s; SA29b =54 sub<strong>culture</strong>s)<br />
Two patterns are noted: Similar migration was obta<strong>in</strong>ed for reference stra<strong>in</strong> WB<br />
SA~ <strong>and</strong> SA24 (arrows) while the ret. stra<strong>in</strong> H7 showed similar migration with lo~al<br />
stra<strong>in</strong>s SA7, SA18, SA29 <strong>and</strong> SA305. Consistent b<strong>and</strong>s were seen for the serial<br />
sub<strong>culture</strong>s <strong>of</strong> the same isolate (SA29).<br />
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