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in vitro culture and isoenzyme analysis of giardia lamblia

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years. Figure 2.1 illustrates age distribution for these samples. The majority <strong>of</strong> the<br />

children (68%) were between the ages 2-5 years. Five <strong>of</strong> the 78 were obta<strong>in</strong>ed from<br />

adults while no ages were <strong>in</strong>dicated <strong>in</strong> the hospital records <strong>in</strong> 9 samples.<br />

Age distribution for Giardia positive<br />

sam pies (from children) retrieved<br />

from the KEH laboratory (n=64)<br />

..<br />

8 0<br />

7 0<br />

60<br />

~ 5 0<br />

.;;; 40<br />

o<br />

~ 30<br />

2 0<br />

1 0<br />

o<br />

0-2 3 m 2 - 5 Y 6 - 1 2 Y<br />

age<br />

1_ % po s itiv e 1<br />

Fig.2.1 Illustrates the percentage age distribution for 64 <strong>of</strong> 78 patients whose faecal<br />

samples (submitted to KEH microbiology laboratory between January 1996 <strong>and</strong><br />

December 1997) were harbour<strong>in</strong>g Giardia. Approximately 69% <strong>of</strong> the patients were<br />

children aged between 2 <strong>and</strong> 5 years.<br />

The percentage <strong>of</strong> Giardia positive samples ranged between 0,36 (July 1996) <strong>and</strong><br />

4,11 (May 1997) as shown <strong>in</strong> Table 2. 4 <strong>and</strong> Giardia was not detected from samples<br />

submitted dur<strong>in</strong>g the months <strong>of</strong> May <strong>and</strong> October 1996. It should be noted, that<br />

many confound<strong>in</strong>g factors <strong>in</strong> this sample population do not allow for determ<strong>in</strong>ation<br />

<strong>of</strong> real prevalence: (i) the submitted samples were obta<strong>in</strong>ed from patients who came<br />

from different geographical areas because KEH is a referral hospital for many faroutly<strong>in</strong>g<br />

areas (ii) the sample population comprised <strong>of</strong> a mixture <strong>of</strong> adults <strong>and</strong><br />

children (iii) direct microscopic screen<strong>in</strong>g is known to be less sensitive than the<br />

concentration methods, therefore real prevalence would be underestimated.<br />

51

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