in vitro culture and isoenzyme analysis of giardia lamblia

(4) 0.2 and 0.3M Tris maleate buffers were prepared similarly, with the appropriate
adjustments in quantities.
(5) 0.3 M Tris Buffer
37.34g/L Tris buffer (Associated Chemical Enterprises cc., Reuven, SA.)
(6) O,3M Tris-Hydrochloride buffer, pH adjusted to 8,0 with 10M NaOH
(7) O,3M Tris-Hydrochloride buffer, pH 7,4
(8) 0,1 M Sodium di-Hydrogen phosphate, pH adjusted to 7,0 with di-Sodium
Hydrogen phosphate
(9) O,2M Sodium hydrogen phosphate / O,03M Boric Acid
(10) O,5M Tris Hydrochloride pH adjusted to 8.0 with 10M NaOH.
(11) L-Aspartic acid (30mM) is dissolved in 0.1 M Tris/HCI buffer and pH adjusted
to 8.0 with KOH
(12) 2-0xoglutaric acid (15mM) is dissolved in 0.1M Tris/Hel buffer and pH
adjusted to 8.0 with KOH
(13) 0, 2M Citrate buffer, pH 7,0
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