- Page 1 and 2: IN VITRO CULTURE AND ISOENZYME ANAL
- Page 3 and 4: (j)edicated to my {ate parents :No
- Page 5: the positive specimens by sucrose g
- Page 9 and 10: ACKNOWLEDGEMENTS The author wishes
- Page 11 and 12: Table of Contents Page CHAPTER 1 -
- Page 13 and 14: 4.2.1. Animal Sourcing and Care ...
- Page 15 and 16: APPENDIX 8 List of enzyme systems u
- Page 17 and 18: List of Tables Page Table 1.1 Commo
- Page 19 and 20: List of Plates Page Plate 2.1 An eo
- Page 21 and 22: ABBREVIATIONS AND UNITS OF MEASUREM
- Page 23 and 24: agi/is in 1882. In 1914, Alexeieff
- Page 25 and 26: unique about Giardia in humans, whi
- Page 27 and 28: To-date, use of the different speci
- Page 29 and 30: Differentiation into the cyst stage
- Page 31 and 32: 1.3 Transmission 1.3.1 Human hosts
- Page 33 and 34: Owen, 1984) 1.3. 2 The Zoonosis of
- Page 35 and 36: 1.4 Distribution The morbidity and
- Page 37 and 38: children by Schutte et al. in 1981
- Page 39 and 40: of (a) the enormous absorptive capa
- Page 41 and 42: Ig M (100% of patients), IgG (70%),
- Page 43 and 44: chymotrypsin) between different iso
- Page 45 and 46: impact on the physical growth of 29
- Page 47 and 48: Figure 1.3. Transmission Electron m
- Page 49 and 50: among children between the ages of
- Page 51 and 52: where immunocompromised mice have p
- Page 53 and 54: up to 4 distinct genotypes within e
- Page 55 and 56: in initiation of infection (excysta
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In order to address these and many
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multiple stool examinations in 4 of
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2.2 MATERIALS AND METHODS 2.2.1 Spe
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Table 2.1. Semi-quantitative assess
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2.2.3.2 The modified Zinc sulphate
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Preliminary work with five samples
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% Viability = unstained cysts/total
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Table 2.3. Prevalence of Giardia in
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Table 2. 4. Monthly summary of stoo
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spp (59%). Other parasites less fre
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Observations • A large proportion
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Plate 2.1. An eosin-stained prepara
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2.4 DISCUSSION In terms of yield, i
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examination of wet preparations. Th
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ecognised (on unstained preparation
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cultivation in vitro (Kasprzak & Ma
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3.2 MATERIALS AND METHODS Three exc
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3.2.3 The modified Acid Pepsin meth
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• After 15 minutes incubation: Ma
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Plate 3.2 (b) A completely excysted
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Twenty-four batches of cysts (71 %
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3.3.3 The modified Acid Pepsin excy
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3.4 DISCUSSION Since our source of
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an unreliable indicator of viabilit
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propagating Giardia for subsequent
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4.2 MATERIALS AND METHODS 4.2.1. An
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A litter comprising of 7 C57BU6 mic
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for attached trophozoites and maint
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days pi. However, accurate quantifi
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Table 4.4. Summary of the inoculati
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not be infected in comparison with
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A proportionately higher percentage
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These findings strongly suggest tha
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CHAPTERS IN VITRO CULTURE OF GIARDI
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Diamond et al.'s (1978) medium. He
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Majewska, 1985) United States (Nash
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were evaluated. 5,2,1,2 Biosate Bio
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Table 5.1 Outlines a relative semi-
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dislodged by chilling the culture t
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Table 5.2. Sensitivity to Ciproflox
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Table 5.3. Summary results of in vi
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Once established in culture for a l
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Plates 5.3 and 5.4 Confluent growth
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difficult to produce viable culture
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dominance of certain genotypes unde
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Cryopreservation and successful ret
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adjusted to 5°C per minute from am
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6.3 RESULTS Trophozoites have been
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Table 6.1. A longevity record of sa
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Diamond (1995) stated that an impor
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into two categories. One group cont
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technique to the first local (South
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for Giardia Iysates. These included
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4. To exclude the presence of bacte
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Plate 7.1 Banding pattern of 8 seri
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PGM activity was displayed by Giard
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Plate 7.6. Hexokinase Lane 1. Unino
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Plate 7.8. Phosphogluconate dehydro
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stably expressed between different
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techniques, which would allow diffe
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Belosevic M, Faubert GM, MacLean JD
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Farthing MJG, Mata L, Urrutia JJ, K
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Heidelberg pp.397-398. Hiatt RA, Ma
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patients with X-linked agammaglobul
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Press, Calgary. pp 57-58. Nash TE,
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of Entamoeba histolytica in a group
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Wolfe M. S.1979. Giardiasis. Sympos
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APPENDICES APPENDIX 1 Information t
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Unayo imvume yokunqaba ukungenela l
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Appendix 3 Excystation and culture
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Appendix 4 Results of excystation a
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Appendix 5 Results of in vitro (aci
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Key to Appendix 5 *Replicated inocu
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Appendix 7 Preparation of culture m
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Appendix 8 List of enzyme systems u
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Appendix 9 Appendix (9a) Preparatio
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Appendix 10. Quantitative descripti