in vitro culture and isoenzyme analysis of giardia lamblia

the bottom of the culture tubes and never showed signs of division. Most of these
gradually began to die until, by day 7, no living trophozoites were seen in the
culture tubes. In 36% of them (Table 5.3), no evidence of contaminants (aerobic
and anaerobic) could be demonstrated.
Sixty-four percent of the cultures were overwhelmed by bacterial contaminants
and died, despite the incorporation of antibiotics in the culture media (Table 5.3).
Some contaminating organisms were identified. Most frequently gas-forming
anaerobes (foul smelling gas evident in the TYI-S-33 culture tubes and growth
only occurred on the c;inaerobic blood plate) contaminated the cultures.
Occasionally, yeasts and Pseudomonas spp were identified as the contaminating
organisms. In some cases Klebsiella and Aerobacter spp were isolated.
No viable cultures were established using the trophozoites obtained from in vitro
excystations.
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