In silico cloning - Bits.vib.be

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Select Use existing enzyme list | Click the Browse for enzyme list button ( ) | Select ’Popular enzymes’ in the Cloning folder under Enzyme lists | click OK Write 3’ into the left filter | Select all enzymes | Click the Add ( ) button
Click Next <strong>In</strong> this step you specify that you want to use enzymes that cut the sequence only once. Deselect the Two restriction sites checkbox | click Next Select Add restriction sites as annotations on sequence | Create restriction map | Finish You see that there are only two enzymes that fulfill these criteria (SacII and KpnI).
Page 1 and 2: Tutorial 1: Primer design In this t
Page 3 and 4: Clicking the dot will select the re
Page 5 and 6: By adjusting the Primer parameters
Page 7 and 8: In the Side Panel of the view conta
Page 9 and 10: Tutorial 2: In silico cloning workf
Page 11: You can do all kinds of restriction
Page 15 and 16: Click OK and the sequence will be i
Page 17 and 18: fine the minimum number of position
Page 19 and 20: Use the Browse button to select pcD
Page 21 and 22: Next step is to cut the fragment. A
Page 23 and 24: Save the construct shown in the cir
Page 25 and 26: Switch to the fragment sequence. Cl
Page 27 and 28: Note that the Workbench is validati
Page 29 and 30: 4.1 Add attB sites The first step i
Page 31 and 32: This will allow you to choose which
Page 33 and 34: You also get a list of primers. Aga
Page 35 and 36: Next you have to specify the entry
Page 37 and 38: When you have selected your entry c

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