In silico cloning - Bits.vib.be

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Now, you can let CLC Main Work<strong>be</strong>nch calculate all the possible primer pairs based on the Primer parameters that you have defined: Click the Calculate button in the Primer Designer Settings This opens the Calculation parameters window: You can modify the parameters of the combination of primers. If you leave them unchanged and you just click 'Calculate' a table is opened showing the possible combinations of primers.
<strong>In</strong> the Side Panel of the view containing the primer pairs you can adjust the Primer Table Settings, to specify the info you want to display in the table, e.g. Fragment length Clicking a primer pair in the table will make a corresponding selection on the sequence in the view. At this point, you can settle on a specific primer pair or save the table for later. If you want to use the first primer pair for your experiment, right-click the primer pair in the table and save the primers.
Page 1 and 2: Tutorial 1: Primer design In this t
Page 3 and 4: Clicking the dot will select the re
Page 5: By adjusting the Primer parameters
Page 9 and 10: Tutorial 2: In silico cloning workf
Page 11 and 12: You can do all kinds of restriction
Page 13 and 14: Click Next In this step you specify
Page 15 and 16: Click OK and the sequence will be i
Page 17 and 18: fine the minimum number of position
Page 19 and 20: Use the Browse button to select pcD
Page 21 and 22: Next step is to cut the fragment. A
Page 23 and 24: Save the construct shown in the cir
Page 25 and 26: Switch to the fragment sequence. Cl
Page 27 and 28: Note that the Workbench is validati
Page 29 and 30: 4.1 Add attB sites The first step i
Page 31 and 32: This will allow you to choose which
Page 33 and 34: You also get a list of primers. Aga
Page 35 and 36: Next you have to specify the entry
Page 37 and 38: When you have selected your entry c

In silico cloning - Bits.vib.be

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