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By adjusting the Primer parameters in the Side Panel you can define primers to meet your needs.<br />
Since the dots are dynamically updated, you can immediately see how a change in the primer<br />
parameters affects the num<strong>be</strong>r of red and green dots.<br />
Remem<strong>be</strong>r: each dot represents the starting point of a primer: you must look for individual green<br />
dots: they represent primers that meet the requirements. You do not need to look for 18<br />
consecutive green dots: one dot represents a whole primer.<br />
1.3 Calculating a primer pair<br />
Until now, we have <strong>be</strong>en looking only at the forward primer. To mark a region for the reverse primer,<br />
make a selection from position 1200 to 1400 and:<br />
Right-click the selection | Reverse primer region here
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