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This will create a new sequence representing the PCR product. Save the sequence in the Cloning<br />
folder and close the views. You do not need to save the fragment table.<br />
2.4 Specify restriction sites and perform <strong>cloning</strong><br />
The final step is to insert the fragment into the <strong>cloning</strong> vector:<br />
Toolbox in the Menu Bar | Cloning and Restriction Sites | Cloning<br />
Select the Fragment (ATP8a1 mRNA (ATP8a1 fwd - ATP8a1 rev)) sequence | click Next