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In silico cloning - Bits.vib.be

In silico cloning - Bits.vib.be

In silico cloning - Bits.vib.be

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Make sure that you circularize the vector and save it <strong>be</strong>fore you proceed with the actual <strong>cloning</strong>.<br />

Toolbox | Cloning and Restriction Sites | Gateway Cloning | Create Entry Clone<br />

Now you can select one or more sequences that will <strong>be</strong> the sequence of interest to <strong>be</strong> recombined<br />

into your entry vector. Note that the sequences you select should <strong>be</strong> flanked with attB sites. You<br />

can select more than one sequence as input, and the corresponding num<strong>be</strong>r of entry clones will <strong>be</strong><br />

created. When you have selected your sequence(s), click Next.

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