In silico cloning - Bits.vib.be

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At the bottom of the view you can now see information about how the vector will be cut open. Since the vector has now been split into two fragments, you can decide which one to use as the target vector. If you selected first the HindIII site and next the XhoI site, the CLC Main Workbench has already selected the right fragment as the target vector. If you click one of the vector fragments, the corresponding part of the sequence will be highlighted.
Next step is to cut the fragment. At the top of the view you can switch between the sequences used for cloning (at this point it says pcDNA4_TO 5.078bp circular vector). Switch to the fragment sequence and perform the same selection of cut sites as before while pressing the Ctrl key. At the bottom of the view you can again see information about how the fragment will be cut. Since the fragment can split into two fragments, you can decide which one to use as the target fragment. If you selected first the HindIII site and next the XhoI site, the CLC Main Workbench has already selected the right fragment. If you click one of the fragments, the corresponding part of the sequence will be highlighted.
Page 1 and 2: Tutorial 1: Primer design In this t
Page 3 and 4: Clicking the dot will select the re
Page 5 and 6: By adjusting the Primer parameters
Page 7 and 8: In the Side Panel of the view conta
Page 9 and 10: Tutorial 2: In silico cloning workf
Page 11 and 12: You can do all kinds of restriction
Page 13 and 14: Click Next In this step you specify
Page 15 and 16: Click OK and the sequence will be i
Page 17 and 18: fine the minimum number of position
Page 19: Use the Browse button to select pcD
Page 23 and 24: Save the construct shown in the cir
Page 25 and 26: Switch to the fragment sequence. Cl
Page 27 and 28: Note that the Workbench is validati
Page 29 and 30: 4.1 Add attB sites The first step i
Page 31 and 32: This will allow you to choose which
Page 33 and 34: You also get a list of primers. Aga
Page 35 and 36: Next you have to specify the entry
Page 37 and 38: When you have selected your entry c

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