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This will add an annotation to this region, Forward primer region, (unselect Show in the Restriction<br />
sites settings in the Side Panel to get a more clear view of the annotations) and 5 rows of red and<br />
green dots (you have to zoom in to see the dots: use the 100% button) representing the primer<br />
suggestions. There is a line for each primer length - 18bp through to 22 bp, hence five lines.<br />
1.2 Examining the primer suggestions<br />
Each line consists of a num<strong>be</strong>r of dots, each representing the starting point of a possible primer,<br />
e.g. the first dot on the first line represents a primer starting at the dot's position and with a length<br />
of 18 nucleotides. The primer will anneal to the selected region in the DNA.<br />
Position the mouse cursor over a dot. A box will appear, providing data about this primer.
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