In silico cloning - Bits.vib.be

More documents

Recommendations

Info

You see that you can use EcoRV to linearize the vector. Toolbox | Coning and Restriction Sites | Cloning Select atp8a1mRNA. Click Next. Select vector pcDNA4-T0. Click Finish. Select EcoRV restriction site.
Switch to the fragment sequence. Click Add as fragment. Click Clone. Click Finish. The vector now contains the complete atp8a1mRNA fragment. You see that the process for working with a signle restriction site is exactly the same as for two restriction sites.
Page 1 and 2: Tutorial 1: Primer design In this t
Page 3 and 4: Clicking the dot will select the re
Page 5 and 6: By adjusting the Primer parameters
Page 7 and 8: In the Side Panel of the view conta
Page 9 and 10: Tutorial 2: In silico cloning workf
Page 11 and 12: You can do all kinds of restriction
Page 13 and 14: Click Next In this step you specify
Page 15 and 16: Click OK and the sequence will be i
Page 17 and 18: fine the minimum number of position
Page 19 and 20: Use the Browse button to select pcD
Page 21 and 22: Next step is to cut the fragment. A
Page 23: Save the construct shown in the cir
Page 27 and 28: Note that the Workbench is validati
Page 29 and 30: 4.1 Add attB sites The first step i
Page 31 and 32: This will allow you to choose which
Page 33 and 34: You also get a list of primers. Aga
Page 35 and 36: Next you have to specify the entry
Page 37 and 38: When you have selected your entry c

In silico cloning - Bits.vib.be

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?