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The Work<strong>be</strong>nch is not doing any primer design. You specify the length of the part of the primers<br />
that should base pair with the ATP8a1 CDS, together with the attB sites and optional primer additions,<br />
and these are the primers. The Work<strong>be</strong>nch will not check the compatibility of the primers, the<br />
annealing temperatures or the GC-content.<br />
Click Next.<br />
Click Finish.<br />
The main output is the input sequence now including attB sites and primer additions. The attB sites,<br />
the primer additions and the primer regions are annotated in the final result. There will <strong>be</strong> one output<br />
sequence for each sequence you have selected for adding attB sites.
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