In silico cloning - Bits.vib.be

fine the minimum number of positions and the number of consecutive positions in the 3’ end
of the primer that must base pair to the sequence in order to speak of a match.
Note that the number of mismatches is reported in the output, so you can filter on this afterwards.
Below the Match criteria, you can adjust Concentrations concerning the reaction mixture. This is
used when reporting melting temperatures for the primers.
Click Next | Adjust the output options
Click Finish
You now see the fragment table displaying the PCR product. In the Side Panel you can choose to
show information about melting temperature for the primers.
Right-click the fragment and select Open Fragment.