In silico cloning - Bits.vib.be

More documents

Recommendations

Info

This will create a new sequence representing the PCR product. Save the sequence in the Cloning folder and close the views. You do not need to save the fragment table. 2.4 Specify restriction sites and perform cloning The final step is to insert the fragment into the cloning vector: Toolbox in the Menu Bar | Cloning and Restriction Sites | Cloning Select the Fragment (ATP8a1 mRNA (ATP8a1 fwd - ATP8a1 rev)) sequence | click Next
Use the Browse button to select pcDNA4_TO cloning vector in the Cloning folder | click OK Click Finish You now see the cloning editor with the pcDNA4_TO vector in a circular view. Press and hold the Ctrl key while you click first the HindIII site and next the XhoI site.
Page 1 and 2: Tutorial 1: Primer design In this t
Page 3 and 4: Clicking the dot will select the re
Page 5 and 6: By adjusting the Primer parameters
Page 7 and 8: In the Side Panel of the view conta
Page 9 and 10: Tutorial 2: In silico cloning workf
Page 11 and 12: You can do all kinds of restriction
Page 13 and 14: Click Next In this step you specify
Page 15 and 16: Click OK and the sequence will be i
Page 17: fine the minimum number of position
Page 21 and 22: Next step is to cut the fragment. A
Page 23 and 24: Save the construct shown in the cir
Page 25 and 26: Switch to the fragment sequence. Cl
Page 27 and 28: Note that the Workbench is validati
Page 29 and 30: 4.1 Add attB sites The first step i
Page 31 and 32: This will allow you to choose which
Page 33 and 34: You also get a list of primers. Aga
Page 35 and 36: Next you have to specify the entry
Page 37 and 38: When you have selected your entry c

In silico cloning - Bits.vib.be

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?