Vol 44 # 2 June 2012 - Kma.org.kw

June 2012
KUWAIT MEDICAL JOURNAL 128
serum ferritin, serum iron, total iron binding capacity,
and hemogram values in blood samples obtained
during the EF and LL phases. Plasma glucose, serum
total iron binding capacity (TIBC) and iron (Fe) was
analyzed using the enzymatic kinetic (hexokinase-
UV/NAD) method on an Olympus AU400 instrument
with Olympus agents. Intra-assay and interassay
Co-efficient Variation (CV) percent respectively
was for glucose < 3.5%, Fe < 4%, TIBC < 2%. Serum
ferritin level was measured by Beckman Coullter
(< 10% CV). Hemogram values were analyzed on
MH6000 (Medpa) (for Plt CV < 6 for others < 5%).
Body Mass Index (BMI) was defined as the body
weight (kilograms) divided by height (meters) 2 .
The categorization of the patients relative to the BMI
was carried out according to the classification of the
World Health Organization (≤ 18.5, underweight; 18.5
- 24.9, normal; 25 - 29.9, overweight; and 30 - 34.9,
Class I obesity) [20] . The individuals with an additional
disease (e.g., anemia, ovarian cyst, thyroid disease,
etc) were excluded from the study.
Statistical Analysis
The statistical analysis was made with SPSS
(Statistical Package for Social Sciences 15.0 version).
The data were analyzed by using number and
percentage distributions, descriptive statistics (mean,
frequency, standard deviation) as well as ANOVA,
Tukey, Kruskal-Wallis, Mann-Whitney U tests and
Paired t-test.
RESULTS
In this study, the mean age of the subjects was 20.21
± 2.76 years. The subjects were categorized based on the
BMI value as follows: 21.2% were underweight, 69.5%
were normal, 7.6% were overweight, and 1.7% were
Class I obese. While 57.6% of the study population
had a PMSS score ≥ 111 points, 42.4% were found to
have a PMSS score < 111 points. The scores of the study
sample with regard to the subdimensions of the PMSS
are shown in Fig. 1.
Hematologic parameters of the study population
during the EF and LL phases are shown in Table
1. No statistically significant difference was found
between the measurements performed before and
after the menstruation with regard to fasting blood
glucose, serum ferritin, serum iron, TIBC , erythrocyte,
monocyte, eosinophil, basophil, hemoglobin, hematocrit
(Hct), thrombocyte, mean corpuscular hemoglobin
(MCH), mean corpuscular hemoglobin concentration
(MCHC), mean corpuscular volume (MCV), and red
blood cell distribution width (RDW) values (p > 0.05).
However, leukocyte and neutrophil values during the
LL phase were observed to be higher than those during
the EF phase, whereas lymphocyte value was found to
be higher during the EF phase compared with the LL
phase (p < 0.001, Table 1). The difference between class
1 obese subjects (PMSS scores:143 ± 19.8) compared
to underweight (PMSS scores:103.92 ± 23.54) and
normal weight (PMSS scores: 122.62 ± 23.54) subjects is
statistically significant (p < 0.05).
Fig. 1: Descriptive statistics of the premenstrual syndrome scale