Biosafety Manual PDF - Lawrence Berkeley National Laboratory
Biosafety Manual PDF - Lawrence Berkeley National Laboratory
Biosafety Manual PDF - Lawrence Berkeley National Laboratory
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<strong>Biosafety</strong> <strong>Manual</strong><br />
IBC-approved version (May 18, 2010)<br />
necessary to prevent contamination of the laboratory with the agents being handled and<br />
contamination of the work with agents from the environment. See Appendix D for common<br />
GMP. Supervisors and work leads are responsible for selecting and instructing workers on the<br />
specific GMP needed to conduct the work, or additional safety practices needed for specific<br />
agents or procedures.<br />
4.2 Safety and Personal Protective Equipment<br />
Worker exposure to infectious agents may be prevented by the use of standard and activityspecific<br />
safety and personal protective equipment (PPE) as primary barriers or controls. The<br />
need for additional activity-specific safety equipment or PPE must be determined during risk<br />
assessment, and any equipment needed for safety should be included in the <strong>Biosafety</strong> Work<br />
Authorization.<br />
Standard safety equipment and PPE are equipment controls listed as BL containment criteria<br />
in BMBL and the NIH Guidelines. They provide primary barriers that prevent worker exposure to<br />
infectious agents. See Section 4.4 of this manual for additional information on BL containment<br />
categories and criteria. These standard equipment requirements, along with requirements from<br />
other biosafety standards, are used and customized for the work to be conducted. They are also<br />
summarized in the LBNL <strong>Biosafety</strong> Work Authorization. Standard safety equipment and PPE are<br />
applicable to most work with biological materials at LBNL. The following types of standard<br />
equipment and PPE are further discussed in Appendix C and Section 5.0 of this manual:<br />
• biosafety cabinets<br />
• PPE<br />
• other physical containment devices such as centrifuge safety cups<br />
4.3 Facility Design and Construction<br />
Facility design and equipment provide secondary barriers that protect laboratory workers,<br />
persons outside the laboratory, the public, and the environment from potentially hazardous<br />
materials or agents that may be accidentally released from the laboratory.<br />
Standard facilites provide secondary barriers.<br />
Source: HHS CDC Office of Health and Safety.<br />
LBNL designs and operates its facilities where work with biological materials is conducted in<br />
accordance with applicable standard facilities criteria. Standard facilities are design features,<br />
materials, and equipment incorporated into the laboratory or facility in accordance with BL<br />
containment criteria stated in BMBL and the NIH Guidelines (see Section 4.4 for more<br />
information).<br />
Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version.<br />
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