Biosafety Manual PDF - Lawrence Berkeley National Laboratory

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Biosafety Manual IBC-approved version (May 18, 2010) • Area-specific PPE requirements must be established for all Technical Areas (e.g., laboratories) and must be posted on the LBNL entrance placard. Minimum PPE for laboratories where biological materials are stored or handled includes safety protective eyewear, long pants, and closed-toe shoes. Area PPE requirements apply to the entire Technical Area unless an exception is granted in accordance with the procedure described in PUB-3000, Chapter 19, Appendix A. • Activity- or operation-specific PPE requirements are assessed and defined in the Biosafety Work Authorization, which covers what PPE must be used (e.g., gloves, laboratory coats, and safety glasses) and any maintenance (e.g., laundering) or disposal requirements. General requirements and conditions for use of PPE related to biosafety include: • The supervisor or work lead is responsible for: o Determining what PPE is required to prevent occupational exposure o Providing at no cost to an employee the PPE required by this section or specified in the Biosafety Work Authorization. This PPE must be readily available in appropriate sizes. o Ensuring that employees and visitors properly use and store required PPE • The EH&S Division is available to assist supervisors or work leads in evaluating work activities and selecting appropriate PPE. • Employees and visitors are responsible for using PPE when required and whenever the work poses a reasonable probability of eye injury or exposure. • In general, removed PPE must be: o Decontaminated when needed, or o Disposed of in accordance with LBNL medical/biohazardous, hazardous, and radiological waste management requirements. • PPE that protects against exposure to BBP materials is considered appropriate if it does not permit BBP material (e.g., human blood or cell culture solution) to pass through the employee's work clothes, street clothes or undergarments, skin, eyes, or other mucous membranes under normal conditions of use and for the duration of time in which the PPE will be worn. 5.4.1 Body Protection Protective laboratory clothing is a garment such as a lab coat, gown, smock, or uniform designed to keep personal clothing, forearms, or other exposed bodily surfaces protected from contamination by biological materials or exposure to other hazards. The term "protective laboratory clothing” typically applies to garments worn in the laboratory, but may also apply to garments worn in nonlaboratory work (e.g., health care). The following biosafety criteria are applicable to wearing protective laboratory clothing: • Protective laboratory clothing should be worn to prevent contamination of personal clothing when working at BL1. • Protective laboratory clothing must be worn when working at BL2 or when working with RG2 or other hazardous materials. This clothing must be removed and left in the laboratory before leaving for nonlaboratory areas (e.g., cafeteria, library, administrative offices). Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 44
Biosafety Manual IBC-approved version (May 18, 2010) Protective laboratory clothing removed after use at BL2 or with biohazardous materials must be handled in one of the following ways: • Placed in a laundry bag or container for cleaning by a qualified laundry service • Disposed of in accordance with LBNL medical/biohazardous, hazardous, and radiological waste management requirements • Stored for reuse if not contaminated. Such clothing stored for reuse should be stored in a manner that would not contaminate other items in case the protective clothing has unknown contamination (e.g., separate coat hook). Protective laboratory clothing and other laundry contaminated with RG2 materials should be handled as noted below, and laundry contaminated with BBP materials must be handled as follows: • Handled as little as possible with a minimum of agitation • Bagged or containerized at the location where it was used but not sorted or rinsed in the location of use • Placed in bags or containers that have biohazard labels, are red in color, or are identified by an alternative laundry labeling or color-coding system that uses universal precautions • Placed and transported in bags or containers that prevent soak-through or leakage of fluids to the exterior if the laundry is sufficiently wet Place protective clothing in properly identified laundry bags. 5.4.2 Eye and Face Protection Eye protection is a safety device such as safety glasses with side shields or goggles worn over the eyes to prevent injury to the eye or exposure to biological agents. Face protection is a safety device such as a face mask, face shield, or other splatter guard worn over all or part of the face to protect the face from injury or exposure to biological agents. Face masks or respirators that are occasionally used for face protection are discussed in Section 5.4.5 of this manual. Eye and face protection is used by laboratory and other workers to protect the eyes and face from splashes, splatters, or flying debris and hand-eye contact with biological materials. Contact by these means may result in injuries to the eyes and face or accidental inoculation via the eyes, nose, or mouth and subsequent infection and disease. The risks noted above are prevented by using eye and face protection in accordance with the following requirements: • As a minimum requirement, safety glasses with side shields must be worn at all times when in a Technical Area such as a laboratory. Area PPE requirements apply to the entire Technical Area unless an exception is granted in accordance with the procedure described in PUB-3000, Chapter 19, Appendix A. Additional eye or face protection may be necessary when handling chemicals or biological materials (e.g., goggles, face shield). Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 45
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