Biosafety Manual PDF - Lawrence Berkeley National Laboratory

More documents

Recommendations

Info

Biosafety Manual IBC-approved version (May 18, 2010) • Eye protection must be worn when conducting procedures that have the potential to create splashes of biological agents, biohazardous materials, or other hazardous materials. • Eye and face protection (e.g., goggles, face mask, face shield, or other splatter guard) must be used when it is anticipated that splashes, sprays, splatters, or droplets of infectious or other hazardous materials may be generated and could contaminate the eyes, nose, or mouth (e.g., when RG2 microorganisms must be handled outside the biosafety cabinet or containment device). This eye and face protection must be included in the Biosafety Work Authorization risk assessment and disposed of with other contaminated laboratory waste or decontaminated before reuse. Eye protection using safety glasses. Source: LBNL EH&S. LBNL provides prescription safety glasses when needed via the EH&S Health Services Group and a staff optometrist. Personnel who need consultation or require prescription safety glasses should schedule an appointment with the optometrist by calling the Health Services Group at (510) 486-6266. 5.4.3 Hand Protection Hand protection is a glove or other safety device used on the hand to prevent injury to the hand or direct skin contact with biological materials. Hand protection is used by laboratory and other workers to protect the hands from harmful physical, chemical, biological, radiological, or other agents or hazards. These agents or hazards may cut, lacerate, abrade, or burn the skin; absorb through the skin; pass through breaks in the skin; or be spread as contamination. Although there are relatively few microbes that can penetrate unbroken skin, there are many circumstances that may cause a break in the skin, such as a cut or puncture from a sharp (see sharps in Section 5.6.6.1). In the case of biological materials, gloves prevent the worker’s hands, fingers, and nails from being contaminated. Spread of biological contamination from the worker’s exposed hands or contaminated gloves to the worker’s mucous membranes or other surfaces may also cause infection and disease in the worker or other people. Glove selection may need to consider protection of the worker from different hazards or serve multiple purposes. For example, gloves used for handling chemical and biological materials may need to be resistant to the chemicals being handled, liquid permeation, and physical damage (see the PPE section of the CHSP). But the remainder of this section is focused only on glove Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 46
Biosafety Manual IBC-approved version (May 18, 2010) criteria that are important for biosafety and gloves that provide protection from biological materials or liquids. The following criteria are applicable to glove selection, use, and disposal: • In general, gloves should be worn to protect the hands from exposure to biological materials or organisms that may present a biological risk. Gloves must be worn to protect hands from exposure to hazardous materials, including: organisms containing recombinant DNA, recombinant experimental animals, RG2 materials, BBP materials or surfaces and items contaminated with BBP materials, when touching mucous membranes and nonintact skin of patients, and when performing vascular access procedures such as phlebotomies. • Glove selection should be based on an appropriate risk assessment. Use of standard nitrile or latex examination gloves is considered adequate for handling most biological materials, and is assumed in the Biosafety Work Authorization. The need for gloves with any additional safety features to handle biological materials should be documented in the Biosafety Work Authorization. The JHA process should be used to assess other hand hazards and glove requirements. • Alternatives to latex gloves should be available because some workers are known to develop allergic reactions to latex. Exposures to latex may result in skin rashes; hives; flushing; itching; nasal, eye, or sinus symptoms; asthma; and (rarely) shock. • When working at BL1 and BL2, workers should remember the following: o Change gloves when contaminated, when their integrity has been compromised, or when otherwise necessary. When working at BL2, wear two pairs of gloves when appropriate. o Remove gloves and wash hands when work with hazardous materials has been completed and before leaving the laboratory. Gloves that were used in BL1 or BL2 work must not be worn outside the laboratory. o Do not wash or reuse disposable gloves. Dispose of used gloves with other contaminated laboratory waste. Hand washing protocols must be rigorously followed. 5.4.4 Foot Protection Footwear appropriate to the worker’s work activities and conditions must be worn at all times. In a Technical Area such as a laboratory, or areas where chemical or biological materials are stored or handled, closed-toe shoes must be worn at all times, and open-toe shoes and sandals are not permitted. In some cases, LBNL requires workers to wear safety shoes for other hazards such as falling heavy objects. See PUB-3000, Section 19.3 (Foot Protection), for additional information. 5.4.5 Respiratory Protection, Respirators, and Face Masks Workers who conduct procedures that may generate aerosols containing harmful levels of infectious agents must use controls such as biosafety cabinets (BSCs), enclosed containment systems, or respirators to avoid inhaling the agents. In general, a BSC should be used as the principal device in laboratories to contain infectious splashes or aerosols generated by numerous microbiological procedures (see Section 5.6.4.2 and Appendix E of this manual for additional BSC information). Other engineered containment devices such as safety centrifuge cups should also be used. When engineering controls are not feasible Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 47
Page 1 and 2: Biosafety Manual Lawrence Berkeley
Page 3 and 4: Biosafety Manual IBC-approved versi
Page 45: Biosafety Manual IBC-approved versi
Page 97 and 98:
Biosafety Manual IBC-approved versi
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187:
show all

Biosafety Manual PDF - Lawrence Berkeley National Laboratory

Create successful ePaper yourself

Delete template?

Save as template?