Biosafety Manual PDF - Lawrence Berkeley National Laboratory

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Biosafety Manual IBC-approved version (May 18, 2010) • Caution placards and other information must be posted at laboratory entrances, including a biohazard label for BL2 work areas. See additional details in the next paragraph. • Consult the Medical and Biohazardous Waste Generator’s Guide (PUB-3095) for details on labels and colors for sharps containers, waste containers, and waste bags in designated red-bag or clear-bag areas. A determination must be made in the Biosafety Work Authorization as to whether the work will generate either regulated medical waste (i.e., red-bag waste that is regulated by the California Department of Health Services) or nonregulated biohazardous waste not (i.e., clear-bag waste). The following criteria must be implemented when posting an entrance to BL1 or BL2 laboratory area: • A Caution placard must be posted at the entrance to a Technical Area as specified in the LBNL CHSP. • Area PPE requirements must be included on the Caution Placard as specified Chapter 19 of PUB-3000 (PPE). • A biohazard label must be posted (typically on a placard) at the entrance to each BL2 work area to advise entering personnel of potential biological hazards. • When infectious agents (i.e., human pathogens) are present or there are organisms that require special provisions for entry (e.g., vaccination), additional biological hazard warning signage is required at the entrance to the laboratory. This signage must incorporate the universal biohazard symbol and include: the laboratory’s biosafety level; the identity of the agent(s) or the words “Infectious Agent(s)”; the name and telephone number of the supervisor, work lead, prinicipal investigator (PI), or other responsible personnel; and any special requirements or procedures for entering and exiting the laboratory. The CHSP Caution Placard will be used to accomplish these additional signage requirements. Any requirements for posting identities of agents or posting special entry and exit procedures will be specified in the BUA. • Other LBNL requirements for signage (e.g., radiological) may also apply to the entrance to be posted. 5.6 Facilities, Laboratory Equipment, and Related Practices This section describes in a topical manner biosafety engineering and work practice controls related to standard facility design and laboratory equipment. Properly designed and used facilities, facility equipment, laboratory equipment, and lab tools provide protection for laboratory workers, persons outside the laboratory, the public, and the environment. See Sections 4.1, 4.2, and 4.3 of this manual for a general discussion of the principles of standard laboratory practices, equipment, and facilities. See Appendix C of this manual for a summary of standard laboratory practices, equipment, and facilities categorized as BL1 and BL2. See PUB-3000, Section 26.7.6, for a discussion of the facility design process at LBNL related to biosafety. 5.6.1 Cleanable Surfaces and Furnishings In general, laboratory facilities and furnishings should be designed and maintained so that they are durable, will not trap contamination, and can be easily cleaned. The following BL1 and BL2 laboratory criteria specified by BMBL and apply to this objective: • The laboratory should be designed so that it can be easily cleaned or decontaminated. Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 50
Biosafety Manual IBC-approved version (May 18, 2010) • Carpets and rugs in laboratories are not permitted. • Laboratory furniture must be capable of supporting anticipated loads and uses. • Spaces between benches, cabinets, and equipment should be accessible for cleaning. • Benchtops must be impervious to water and resistant to heat, organic solvents, acids, alkalis, and other chemicals. • Chairs used in laboratory work must be covered with a nonporous material that can be easily cleaned and decontaminated with an appropriate disinfectant. BMBL, fifth edition, added the new requirement noted above for chairs used in BL1 laboratory work. This new requirement for BL1 work involves significant costs to replace or modify chairs covered with porous material (e.g., cloth or mesh cushions). LBNL divisions may develop and document a corrective action that specifies a phase-in period to replace chairs with porous cushions used in BL1 laboratory work. During any phase-in period, new chairs used in BL1 laboratory work must meet the requirement to be covered with a nonporous material. 5.6.2 Doors and Windows Laboratory doors and windows provide a means to control personnel access to the laboratory, control vectors such as insects and rodents, and maintain laboratory air-flow balance. These controls are elements of standard BL1 or BL2 laboratory practices or facilities. The following biosafety criteria from BMBL and Appendix C are applicable to laboratory doors and windows: • BL1 and BL2 laboratories should have doors for access control. BL2 laboratory doors should be self-closing and have locks in accordance with LBNL standards. When the laboratory is unoccupied during nonbusiness hours, access to the laboratory should be controlled (e.g., by locking doors to the laboratory areas and/or doors to the building entrance). • BL1 laboratory windows that open to the exterior should be fitted with screens. BL2 laboratory windows that open to the exterior are not recommended. However, if a BL2 laboratory has windows that open to the exterior, they must be fitted with screens. 5.6.3 Plumbing Systems and Equipment Plumbing-related systems and equipment that have requirements related to biosafety include handwashing sinks, sanitary sewer drains, water systems and backflow protection, emergency eyewash and shower units, and pipes. These systems provide needed utilities and containment when used properly. When used incorrectly, these systems may provide a route of exposure to personnel or the environment. 5.6.3.1 Sinks and Handwashing BL1 and BL2 laboratories must have a sink with running water for handwashing. In BL2 laboratories, the sink should be located near the exit door and may be manually, hands-free, or automatically operated. Handwashing sinks should be provided with a soap dispenser and paper towel dispenser as a best management practice. When working with BBP materials, the sink facility is called a handwashing facility. A handwashing facility must have an adequate supply of potable running water, soap, and single-use towels or hot-air drying machines. Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 51
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